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Western blot detection of Transferrin in (lane 1) molecular weight, (lane 2) anti-Transferrin (green), Goat-anti-Alpha-1-Anti-Trypsin, and mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Samples were probed using a Transferrin polyclonal antibody (Product # PA1-27251) at a dilution of 1:1000 for overnight at 4°C. Secondary detection was performed using a DyLight 549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10,000 for 45 min at room temperature. Samples were blocked with 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Transferrin isolated from human serum.|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml PEG|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunomicroscopy (IM)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27251 detects Transferrin from human samples.
PA1-27251 has been successfully used in ELISA, immunohistochemistry, immunomicroscopy, and Western blot procedures.
The PA1-27251 immunogen is Transferrin isolated from human serum.
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1,800 to 2,700 mg/L. It is systhesised in the liver and consists of two domains each having a high affinity reversable binding site for Fe3+. The iron is transported in blood and and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin systhesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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