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Invitrogen
Transferrin Polyclonal Antibody
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FIGURE: 1 / 2
Transferrin Antibody (PA1-9525) in WB
Western blot was performed using Anti-Transferrin Polyclonal Antibody (Product # PA1-9525) and a 80 kDa band corresponding to Transferrin was observed across the samples except in HeLa and HeLa conditioned media in Fig. a and was also observed in Hep G2 cells upon treatment with secretion blockers (FLI-06 and PTI) in Fig. b. The expression of Transferrin was not observed in K-562 as reported in literature. An additional uncharacterized band was observed at ~35 kDa across all the samples tested. Whole cell extracts (30ug lysate) of Hep G2 (... View More
Product Details
PA1-9525
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues 30-41, 292-303, and 422-431 of human transferrin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
IgY fraction
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-9525 detects Transferrin in human samples.
PA1-9525 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9525 detects a ~75 kDa protein representing Transferrin.
The PA1-9525 immunogen is a mix of synthetic peptide corresponding to residues 30-41, 292-303, and 422-431 of human transferrin.
Target Information
Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-1 metal-binding globulin; DKFZp781D0156; epididymis secretory sperm binding protein Li 71p; Serotransferrin; Siderophilin; transferin; Transferrin
Gene Aliases: HEL-S-71p; PRO1400; PRO1557; PRO2086; TF; TFQTL1
UniProt ID: (Human) P02787
Entrez Gene ID: (Human) 7018
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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