|Tested species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to residues 30-41, 292-303, and 422-431 of human transferrin.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:1000 - 1:5000|
|Western Blot (WB)||1:1000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9525 detects Transferrin in human samples.
PA1-9525 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9525 detects a ~75 kDa protein representing Transferrin.
The PA1-9525 immunogen is a mix of synthetic peptide corresponding to residues 30-41, 292-303, and 422-431 of human transferrin.
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.