Immunofluorescent analysis of Transferrin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Transferrin polyclonal antibody (Product # PA3-913) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human transferrin isolated from plasma.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA3-913 detects transferrin from human and mouse samples.
PA3-913 has been successfully used in Western Blot and ICC/IF procedures.
The PA3-913 immunogen is human transferrin isolated from plasma.
Transferrin is a blood plasma protein that is used in iron ion delivery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neuropathology of sporadic Parkinson disease before the appearance of parkinsonism: preclinical Parkinson disease.
PA3-913 was used in immunohistochemistry to investigate the changes of neuronal system in pre-symptomatic sporadic Parkinson disease
|Ferrer I,Martinez A,Blanco R,Dalfó E,Carmona M||Journal of neural transmission (Vienna, Austria : 1996) (118:821)||2011|