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Agrisera
Tubulin beta chain Polyclonal Antibody
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FIGURE: 1 / 2
Tubulin beta chain Antibody (AS10-681) in ICC/IF
(A) The 3 days old suspension cells of Arabidopsis thaliana have been fixed for 25 minutes with 2% FA, cell wall was partially digested with 0.2% Dricelase in MES buffer (p H 5.1) for 30 min at 37°C and the cells were dried on coated slide to omit PM extraction. Following by incubation for 45 minutes with Agrisera anti-tubulin beta chain primary antibody in a dilution of 1:1,000 washing once and 45 min incu... View More
Product Details
AS10-681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Algae,
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta tubulin; beta tubulin 1; beta-4 tubulin; beta-tubulin; beta-tubulin 1; beta-tubulin 2; beta-tubulin 3; beta-tubulin 4; beta-tubulin 5; beta-tubulin 6; beta-tubulin 7; beta-tubulin 8; beta-tubulin 9; dJ40E16.7; M40 antibody; TUB; TUB1; TUB2; TUB3; TUB4; TUB5; TUB6; TUB7; TUB8; TUB9; TUBB; tubulin beta; tubulin beta chain
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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