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|Tested species reactivity||Tag|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant denatured TurboGFP and non-denatured CopGFP comprising 6XHis Tag|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS/BBS, pH 7.4, with 0.1% dextran, 0.1% mannitol|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:30,000 - 1:50,000|
|Immunocytochemistry (ICC)||1:5,000 - 1:10,000|
|Western Blot (WB)||1:5000 - 1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-22688 can be used to recognize denatured and non-denatured TurboGFP and CopGFP in immunocytochemistry, Western blot, and ELISA assays.
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda)
Reconstitute with sterile water or 50% glycerol to a concentration of 1 mg/ml. Store at -20 C for one year; reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months; reconstituted with 50% glycerol, antibody can be stored at -20°C for six months.
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytocidal activities of topoisomerase 1 inhibitors and 5-azacytidine against pheochromocytoma/paraganglioma cells in primary human tumor cultures and mouse cell lines.
PA5-22688 was used in western blot to study the therapeutic potential of topoisomerase inhibitors and 5-azacytidine aginst metastatic pheochromocytoma and paraganglioma
|Powers JF,Korgaonkar PG,Fliedner S,Giubellino A,Pacak K,Sahagian GG,Tischler AS||PloS one (9:null)||2014|
GFP2, Green Fluoresecent Protein, GFP, Turbo GFP, CopGFP, ppluGFP2