Immunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red).
|Tested species reactivity||Tag|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant denatured TurboGFP and non-denatured CopGFP comprising 6XHis Tag|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS/BBS, pH 7.4, with 0.1% dextran, 0.1% mannitol|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:30,000 - 1:50,000|
|Immunocytochemistry (ICC)||1:5,000 - 1:10,000|
|Western Blot (WB)||1:5000 - 1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22688 can be used to recognize denatured and non-denatured TurboGFP and CopGFP in immunocytochemistry, Western blot, and ELISA assays.
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda)
Reconstitute with sterile water or 50% glycerol to a concentration of 1 mg/ml. Store at -20 C for one year; reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months; reconstituted with 50% glycerol, antibody can be stored at -20°C for six months.
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly.
PA5-22688 was used in immunocytochemistry to investigate how early embryonic-like cells are promoted by downregulating replication-dependent chromatin assembly
|Ishiuchi T,Enriquez-Gasca R,Mizutani E,Bo¿kovi¿ A,Ziegler-Birling C,Rodriguez-Terrones D,Wakayama T,Vaquerizas JM,Torres-Padilla ME||Nature structural and molecular biology (22:662)||2015|
|Not Applicable||Not Cited||
DNA damage induces GDNF secretion in the tumor microenvironment with paracrine effects promoting prostate cancer treatment resistance.
PA5-22688 was used in western blot to study how glial cell line-derived neurotrophic factor promotes prostate cancer growth.
|Huber RM,Lucas JM,Gomez-Sarosi LA,Coleman I,Zhao S,Coleman R,Nelson PS||Oncotarget (6:2134)||2015|
Cytocidal activities of topoisomerase 1 inhibitors and 5-azacytidine against pheochromocytoma/paraganglioma cells in primary human tumor cultures and mouse cell lines.
PA5-22688 was used in western blot to study the therapeutic potential of topoisomerase inhibitors and 5-azacytidine aginst metastatic pheochromocytoma and paraganglioma
|Powers JF,Korgaonkar PG,Fliedner S,Giubellino A,Pacak K,Sahagian GG,Tischler AS||PloS one (9:null)||2014|