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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • U2AF1 Antibodies

          Invitrogen

          U2AF1 Polyclonal Antibody

          1 Published Figure
          1 Reference
          View all (10) U2AF1 antibodies

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          Cite U2AF1 Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (1)
          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
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          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          U2AF1 Antibody (PA5-28510) in ICC/IF

          Immunocytochemistry analysis of U2AF1 in paraformaldehyde-fixed HeLa. Samples were incubated with U2AF1 polyclonal antibody (Product # PA5-28510) using a dilution of 1:200. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          U2AF1 Antibody in Immunocytochemistry (ICC/IF)
          U2AF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          U2AF1 Antibody in Western Blot (WB)
          U2AF1 Antibody in Immunoprecipitation (IP)
          U2AF1 Antibody in Western Blot (WB)

          Product Details

          PA5-28510

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -

          Immunoprecipitation (IP)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.34 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2545986

          Product Specific Information

          Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

          Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%), Chicken (98%), Bovine (98%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (U2AF),35 kDa; U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein

          View more View less

          Gene Aliases: 2010107D16Rik; 35kDa; FP793; RN; RNU2AF1; U2AF1; U2AF35; U2AFBP

          View more View less

          UniProt ID: (Human) Q01081, (Mouse) Q9D883

          View more View less

          Entrez Gene ID: (Human) 7307, (Mouse) 108121

          View more View less

          Function(s)
          nucleotide binding protein binding poly(A) RNA binding metal ion binding nucleic acid binding RNA binding RS domain binding RNA splicing factor
          Process(es)
          mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA processing RNA export from nucleus mRNA export from nucleus RNA splicing mRNA 3'-end processing regulation of mitophagy positive regulation of protein targeting to mitochondrion
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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