Immunofluorescent analysis of U2AF1L4 showing staining in the nucleus of HCT116 cells. HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a U2AF1L4 polyclonal antibody (Product # PA5-36004) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 202 of U2AF1L4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.