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Invitrogen
U2AF2 Polyclonal Antibody
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FIGURE: 1 / 3
U2AF2 Antibody (PA5-102524) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded U2AF2 in rat liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with U2AF2 polyclonal antibody (Product # PA5-102524) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-102524
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human U2AF2(Accession P26368), corresponding to amino acid residues D272-G322.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total U2AF2.
Target Information
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hU2AF(65); hU2AF65; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2; U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), 65 kDa; U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit
Gene Aliases: 65kDa; DEVDFB; U2AF2; U2AF65
UniProt ID: (Human) P26368, (Mouse) P26369
Entrez Gene ID: (Human) 11338, (Rat) 308335, (Mouse) 22185
spliceosomal complex assembly
mRNA splicing, via spliceosome
mRNA processing
RNA splicing
negative regulation of protein ubiquitination
positive regulation of RNA splicing
negative regulation of mRNA splicing, via spliceosome
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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