Proteintech

UBA6 Polyclonal Antibody

Product Image Gallery

UBA6 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

UBA6 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Western Blot (WB)

1:500-1:1000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UBA6 Fusion Protein Ag4000

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody recognizes the full-length (118 kDa) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Target Information

UBA6 (UBE1L2, MOP-4) is a novel E1 enzyme specific for ubiquitin and belongs to the ubiquitin-activating E1 family. It is a part of the cascade to attach ubiquitin (Ub) covalently to substrate proteins. UBA6 activates ubiquitin by first adenylating its carboxy-terminal glycine residue with ATP and then linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Its isoforms influence spermatogenesis and male fertility. UBA6 is highly expressed in human monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E1-L2; Monocyte protein 4; MOP-4; UBA6, ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; Ubiquitin-activating enzyme E1-like protein 2; ubiquitin-like modifier activating enzyme 6; Ubiquitin-like modifier-activating enzyme 6

Gene Aliases: 4930542H01; 5730469D23Rik; AU021846; AW124799; E1-L2; MOP-4; MOP4; RGD1308324; UBA6; UBE1L2

UniProt ID: (Human) Q86T78, (Mouse) Q8C7R4

Entrez Gene ID: (Human) 55236, (Rat) 305268, (Mouse) 231380

Molecular Function: ligase transfer/carrier protein