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UBA6 Polyclonal Antibody

View all (12) UBE1L2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (7)

UBA6 Antibody in Immunocytochemistry (ICC/IF)

UBA6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

UBA6 Antibody (13211-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

UBA6 Antibody in Immunocytochemistry (ICC/IF)

UBA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UBA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UBA6 Antibody in Western Blot (WB)

UBA6 Antibody in Western Blot (WB)

UBA6 Antibody in Western Blot (WB)

UBA6 Antibody in Western Blot (WB)

Product Image Gallery

UBA6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 13211-1-AP (UBA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

UBA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Mouse testis tissue slide using (Product # 13211-1-AP) UBA6 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Mouse testis tissue slide using (Product # 13211-1-AP) UBA6 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBA6 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with (Product # 13211-1-AP) UBA6 antibody at dilution of 1:2,500 incubated at room temperature for 1.5 hours.

UBA6 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

UBA6 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 13211-1-AP (UBA6 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

UBA6 Antibody in Western Blot (WB) — WB result of UBA6 antibody (13211-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA6 transfected HEK-293 cells.

Product Details

13211-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:500

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UBA6 Fusion Protein Ag4000 (87-389 aa encoded by BC031637)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the full-length (118 kDa) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Immunogen sequence: AVTIHDTEK CQAWDLGTNF FLSEDDVVNK RNRAEAVLKH IAELNPYVHV TSSSVPFNET TDLSFLDKYQ CVVLTEMKLP LQKKINDFCR SQCPPIKFIS ADVHGIWSRL FCDFGDEFEV LDTTGEEPKE IFISNITQAN PGIVTCLENH PHKLETGQFL TFREINGMTG LNGSIQQITV ISPFSFSIGD TTELEPYLHG GIAVQVKTPK TVFFESLERQ LKHPKCLIVD FSNPEAPLEI HTAMLALDQF QEKYSRKPNV GCQQDSEELL KLATSISETL EEKVTIEIYG CPNICLLIHK CSVY (87-389 aa encoded by BC031637)

Target Information

UBA6 (UBE1L2, MOP-4) is a novel E1 enzyme specific for ubiquitin and belongs to the ubiquitin-activating E1 family. It is a part of the cascade to attach ubiquitin (Ub) covalently to substrate proteins. UBA6 activates ubiquitin by first adenylating its carboxy-terminal glycine residue with ATP and then linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Its isoforms influence spermatogenesis and male fertility. UBA6 is highly expressed in human monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Inhibition of UBA6 by inosine augments tumour immunogenicity and responses.

Nature communications

Zhang L,Jiang L,Yu L,Li Q,Tian X,He J,Zeng L,Yang Y,Wang C,Wei Y,Jiang X,Li J,Ge X,Gu Q,Li J,Wu D,Sadler AJ,Yu D,Xu D,Gao Y,Yuan X,He B

Thu Sep 15 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: E1-L2; Monocyte protein 4; MOP-4; UBA6, ubiquitin-activating enzyme E1; Ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; Ubiquitin-activating enzyme E1-like protein 2; ubiquitin-like modifier activating enzyme 6; Ubiquitin-like modifier-activating enzyme 6

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Gene Aliases: 4930542H01; 5730469D23Rik; AU021846; AW124799; E1-L2; MOP-4; MOP4; UBA6; UBE1L2

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UniProt ID: (Human) A0AVT1, (Mouse) Q8C7R4

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Entrez Gene ID: (Human) 55236, (Mouse) 231380

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ubiquitin activating enzyme activity protein binding ATP binding FAT10 activating enzyme activity nucleotide binding small protein activating enzyme activity ligase activity ubiquitin-protein ligase

ubiquitin-dependent protein catabolic process learning locomotory behavior protein ubiquitination amygdala development hippocampus development protein ubiquitination involved in ubiquitin-dependent protein catabolic process dendritic spine development cellular protein modification process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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