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Invitrogen
UBE2B Polyclonal Antibody
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FIGURE: 1 / 2
UBE2B Antibody (PA5-30200) in WB
Western Blot analysis of UBE2B was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a UBE2B Polyclonal Antibody (Product # PA5-30200) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-30200
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Amount
102 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: mouse brain, DDDDK-tagged UBE2B-transfected 293T.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2 protein; E2 ubiquitin-conjugating enzyme B; E214K; HHR6B (Human homologue of yeast RAD 6); putative; HR6B; RAD6 homolog B; UBE2B; Ubiquitin carrier protein B; ubiquitin conjugating enzyme E2B; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2B, RAD6 homology; Ubiquitin-protein ligase B; unnamed protein product
Gene Aliases: 2610301N02Rik; E2-14k; E2-17kDa; HHR6B; HR6B; mHR6B; RAD6B; UBC2; UBCH1; UBE2B
UniProt ID: (Human) P63146, (Mouse) P63147
Entrez Gene ID: (Human) 7320, (Mouse) 22210
protein polyubiquitination
in utero embryonic development
DNA repair
postreplication repair
chromatin organization
ubiquitin-dependent protein catabolic process
apoptotic process
cellular response to DNA damage stimulus
spermatogenesis
sperm axoneme assembly
response to xenobiotic stimulus
response to UV
positive regulation of reciprocal meiotic recombination
protein ubiquitination
ectopic germ cell programmed cell death
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
meiotic telomere clustering
protein stabilization
chiasma assembly
negative regulation of developmental process
protein autoubiquitination
synaptonemal complex organization
protein K63-linked ubiquitination
protein K48-linked ubiquitination
protein K11-linked ubiquitination
positive regulation of canonical Wnt signaling pathway
negative regulation of post-translational protein modification
negative regulation of reproductive process
maintenance of chromatin silencing
protein monoubiquitination
histone ubiquitination
regulation of histone modification
negative regulation of histone phosphorylation
histone H2A ubiquitination
response to drug
negative regulation of cAMP-mediated signaling
canonical Wnt signaling pathway
histone lysine demethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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