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          • Primary Antibodies ›
          • UBE2D2 Antibodies

          Invitrogen

          UBE2D2 Polyclonal Antibody

          1 Reference
          View all (25) UBE2D2 antibodies

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          Cite UBE2D2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          UBE2D2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          UBE2D2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          UBE2D2 Antibody (PA5-30968) in WB

          Western Blot using UBE2D2 Polyclonal Antibody (Product # PA5-30968). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: U87-MG . 15% SDS PAGE. UBE2D2 Polyclonal Antibody (Product # PA5-30968) diluted at 1:1,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          UBE2D2 Antibody in Western Blot (WB)
          UBE2D2 Polyclonal Antibody

          Product Details

          PA5-30968

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Full length human UBE2D2 Recombinant protein.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 1% BSA, 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2548442

          Product Specific Information

          Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7.

          Predicted reactivity: Rat (100%), Zebrafish (98%), Xenopus laevis (92%), Pig (98%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme D2; E2 ubiquitin-conjugating enzyme D2; p53-regulated ubiquitin-conjugating enzyme 1; similar to UBC4/5 in yeast; Ubiquitin carrier protein D2; ubiquitin conjugating enzyme E2D 2; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); Ubiquitin-protein ligase D2; unnamed protein product

          View more View less

          Gene Aliases: E2(17)KB2; PUBC1; UBC4; UBC4/5; UBC5B; UBCH4; UBCH5B; UBE2D2

          View more View less

          UniProt ID: (Human) P62837

          View more View less

          Entrez Gene ID: (Human) 7322

          View more View less

          Function(s)
          nucleotide binding ubiquitin-protein transferase activity protein binding ATP binding transferase activity ubiquitin protein ligase activity ubiquitin conjugating enzyme activity
          Process(es)
          protein polyubiquitination ubiquitin-dependent protein catabolic process protein ubiquitination protein modification process protein autoubiquitination protein K48-linked ubiquitination
          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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