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UBE2O Antibody (PA5-31429) in IHC (P)
UBE2O Polyclonal Antibody detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O Polyclonal Antibody (Product # PA5-31429) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-31429
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 822 and 1035 of Human UBE2O
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548903
Product Specific Information
Recommended positive controls: 293T, HeLa.
Predicted reactivity: Mouse (95%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: (E3-independent) E2 ubiquitin-conjugating enzyme; (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O; E2/E3 hybrid ubiquitin-protein ligase UBE2O; Ubiquitin carrier protein O; ubiquitin conjugating enzyme E2O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2O; Ubiquitin-protein ligase O; ubiquitinating enzyme E2-230 kDa
Gene Aliases: 9630022H21; B230113M03Rik; E2-230K; KIAA1734; mKIAA1734; UBE2O
UniProt ID: (Human) Q69YP4, (Mouse) Q6ZPJ3
Entrez Gene ID: (Human) 63893, (Mouse) 217342
ubiquitin-protein transferase activity
protein binding
ATP binding
ubiquitin protein ligase binding
poly(A) RNA binding
ubiquitin protein ligase activity
ubiquitin conjugating enzyme activity
nucleotide binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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