Proteintech

UBE2S Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

UBE2S Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14115-1-AP (UBE2S antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

UBE2S Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14115-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UBE2S Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (14115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

UBE2S Antibody in Immunoprecipitation (IP) — IP result of anti-UBE2S (IP:14115-1-AP, 4ug; Detection:14115-1-AP 1:1000) with A2780 cells lysate 960ug.

Product Details

14115-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Flow Cytometry (Flow)	0.5 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	UBE2S Fusion Protein Ag5264 (1-222 aa encoded by BC066948) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MNSNVENLP PHIIRLVYKE VTTLTADPPD GIKVFPNEED LTDLQVTIEG PEGTPYAGGL FRMKLLLGKD FPASPPKGYF LTKIFHLNVG ANGEICVNVL KRDWTAELGI RHVLLTIRCL LIHPNPESAL NEEAGRLLLE NSEEYAARAR LLTEIHGGAG GPSGRAEAGR ALASGTAASS TDPGAPGGLG GAEGPMAKKH AGERDKKLAA KKKTDKKRAL RRL (1-222 aa encoded by BC066948)

Target Information

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.

Biochemical and biophysical research communications

Yoshimura S,Kasamatsu A,Nakashima D,Iyoda M,Kasama H,Saito T,Takahara T,Endo-Sakamoto Y,Shiiba M,Tanzawa H,Uzawa K

Sat Apr 15 00:00:00 EDT 2017

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.

Biochemical and biophysical research communications

Yoshimura S,Kasamatsu A,Nakashima D,Iyoda M,Kasama H,Saito T,Takahara T,Endo-Sakamoto Y,Shiiba M,Tanzawa H,Uzawa K

Sat Apr 15 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: E2 ubiquitin-conjugating enzyme S; E2-EPF; E2-EPF5; Ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S

Gene Aliases: 0910001J09Rik; 6720465F12Rik; AA409170; E2-EPF; E2EPF; EPF5; OK/SW-cl.73; RGD1564746; UBE2S

UniProt ID: (Human) Q16763, (Rat) B5DFI8, (Mouse) Q921J4

Entrez Gene ID: (Human) 27338, (Rat) 292588, (Mouse) 77891

Molecular Function: ubiquitin-protein ligase