Invitrogen

UBE2S Polyclonal Antibody

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UBE2S Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of UBE2S in formalin-fixed paraffin embedded sections human lymph node tissue sections using UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:100.

UBE2S Antibody in Western Blot (WB) — Western Blot analysis of UBE2S using UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:500. Lane A: 293T whole cell lysate, Lane B: HeLa whole cell lysate, Lane C: Jurkat whole cell lysate, Lane D: U-251 MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.

UBE2S Antibody in Immunoprecipitation (IP) — Immunoprecipitation of UBE2S was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunoprecipitation (IP)

1-5 µL/mg lysate

Western Blot (WB)

1:500-1:2,000

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human UBE2S fragment

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.03% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2867032

Target Information

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2 ubiquitin-conjugating enzyme S; E2-EPF; E2-EPF5; Ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S

Gene Aliases: E2-EPF; E2EPF; EPF5; OK/SW-cl.73; UBE2S

UniProt ID: (Human) Q16763

Entrez Gene ID: (Human) 27338

Function(s)