Invitrogen

UBE2S Polyclonal Antibody

View all (27) UBE2S antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

UBE2S Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of UBE2S in formalin-fixed paraffin embedded sections human lymph node tissue sections using UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:100.

UBE2S Antibody in Western Blot (WB) — Western Blot analysis of UBE2S using UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:500. Lane A: 293T whole cell lysate, Lane B: HeLa whole cell lysate, Lane C: Jurkat whole cell lysate, Lane D: U-251 MG whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.

UBE2S Antibody in Immunoprecipitation (IP) — Immunoprecipitation of UBE2S was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of UBE2S Polyclonal Antibody (Product # PA5-112296) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 26 kDa.

Product Details

PA5-112296

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunoprecipitation (IP)	1-5 µL/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human UBE2S fragment
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2867032

Target Information

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: E2 ubiquitin-conjugating enzyme S; E2-EPF; E2-EPF5; Ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2S; Ubiquitin-protein ligase S

Gene Aliases: E2-EPF; E2EPF; EPF5; OK/SW-cl.73; UBE2S

UniProt ID: (Human) Q16763

Entrez Gene ID: (Human) 27338

Molecular Function: ubiquitin-protein ligase