The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins. Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4. In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.
Protein Aliases: RING-type E3 ubiquitin transferase E4 A; Ubiquitin conjugation factor E4 A; ubiquitination factor E4A (UFD2 homolog, yeast); ubiquitination factor E4A, UFD2 homolog
Gene Aliases: 4732444G18Rik; 9930123J21Rik; Ab2-232; E4; KIAA0126; UBE4A; UBOX2; UFD2; UFD2b
Molecular Function: ubiquitin-protein ligase