Invitrogen

UBR4 Polyclonal Antibody

Product Image Gallery

UBR4 Antibody in Western Blot (WB) — Western blot analysis of UBR4 in HeLa cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-116101).

UBR4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of UBR4 in mouse muscle tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116101) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human UBR4, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 150mM NaCl, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Mediates ubiquitination of ACLY, leading to its subsequent degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 600 kDa retinoblastoma protein-associated factor; E3 ubiquitin-protein ligase UBR4; N-recognin-4; p600; RBAF600; retinoblastoma-associated factor 600; retinoblastoma-associated factor 600-like protein; Retinoblastoma-associated factor of 600 kDa; RING-type E3 ubiquitin transferase UBR4; Zinc finger UBR1-type protein 1; zinc finger, UBR1 type 1; ZUBR1

Gene Aliases: 1810009A16Rik; A930005E13Rik; D930005K06Rik; Gm1032; Gm1666; KIAA0462; KIAA1307; mKIAA0462; N28143; p600; RBAF600; UBR4; ZUBR1

UniProt ID: (Human) Q5T4S7, (Mouse) A2AN08, (Rat) Q2TL32

Entrez Gene ID: (Human) 23352, (Mouse) 69116, (Rat) 313658

Function(s)

viral process protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Process(es)