Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of L6 (Lane 1) and C2C12 (Lane 2). The blot was probed with Anti-UCP3 Rabbit Polyclonal Antibody (Product # PA1-055, 1 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution). A 34 kDa band corresponding to UCP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE and reg ; 12% Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Dog, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UCP-3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.
PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.
The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.
The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP-1 is abundantly expressed in brown adipose tissue of mammals. UCP-1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP-2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP-2 to be expressed by a variety of tissues. UCP-3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
4-Hydroxynonenal induces Nrf2-mediated UCP3 upregulation in mouse cardiomyocytes.
PA1-055 was used in western blot to study the role of Nrf2 when mouse cardiomyocytes are treated with 4-Hydroxy-2-nonenal
|López-Bernardo E,Anedda A,Sánchez-Pérez P,Acosta-Iborra B,Cadenas S||Free radical biology and medicine (88:427)||2015|
NF1 is a critical regulator of muscle development and metabolism.
PA1-055 was used in western blot to study the role of NF1 in the development and metabolism of muscle
|Sullivan K,El-Hoss J,Quinlan KG,Deo N,Garton F,Seto JT,Gdalevitch M,Turner N,Cooney GJ,Kolanczyk M,North KN,Little DG,Schindeler A||Human molecular genetics (23:1250)||2014|
Cardiac-specific adipose triglyceride lipase overexpression protects from cardiac steatosis and dilated cardiomyopathy following diet-induced obesity.
PA1-055 was used in western blot to study the protective effects against cardiac steatosis and dilated cardiomyopathy of specifically overexpressing triglyceride lipase in cardiomyocytes in a murine model of diet-induced obesity
|Pulinilkunnil T,Kienesberger PC,Nagendran J,Sharma N,Young ME,Dyck JR||International journal of obesity (2005) (38:205)||2014|
A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes.
PA1-055 was used in western blot to study the regulation of brown adipocyte UCP3 transcription by a novel intronic enhancer that binds SP1 and SP3
|Hoffmann C,Zimmermann A,Hinney A,Volckmar AL,Jarrett HW,Fromme T,Klingenspor M||PloS one (8:null)||2014|
Divergent skeletal muscle respiratory capacities in rats artificially selected for high and low running ability: a role for Nor1?
PA1-055 was used in western blot to study the potential role of Nor1 in the different metabolic profiles and capacities of rats with high and low running abilities
|Stephenson EJ,Stepto NK,Koch LG,Britton SL,Hawley JA||Journal of applied physiology (Bethesda, Md. : 1985) (113:1403)||2012|
Dietary supplementation with vitamin E and C attenuates dexamethasone-induced glucose intolerance in rats.
PA1-055 was used in western blot to study the effect of vitamin E and C on dexamethasone-induced glucose intolerance in rats
|Williams DB,Wan Z,Frier BC,Bell RC,Field CJ,Wright DC||American journal of physiology. Regulatory, integrative and comparative physiology (302:R49)||2012|
Transforming growth factor ß¿ oppositely regulates the hypertrophic and contractile response to ß-adrenergic stimulation in the heart.
PA1-055 was used in western blot to investigate the role of TGF-beta 1 in heart response to beta-epinephrine
|Huntgeburth M,Tiemann K,Shahverdyan R,Schlüter KD,Schreckenberg R,Gross ML,Mödersheim S,Caglayan E,Müller-Ehmsen J,Ghanem A,Vantler M,Zimmermann WH,Böhm M,Rosenkranz S||PloS one (6:null)||2011|
PPAR¿ agonists have opposing effects on insulin resistance in high fat-fed rats and mice due to different metabolic responses in muscle.
PA1-055 was used in western blot to investigate the different effect of PPARdelta agonist on diabetic treatment between rats and mice
|Ye JM,Tid-Ang J,Turner N,Zeng XY,Li HY,Cooney GJ,Wulff EM,Sauerberg P,Kraegen EW||British journal of pharmacology (163:556)||2011|
Effect of LKB1 deficiency on mitochondrial content, fibre type and muscle performance in the mouse diaphragm.
PA1-055 was used in western blot to investigate the role of LKB1 in skeletal muscle metabolic processes
|Brown JD,Hancock CR,Mongillo AD,Benjamin Barton J,DiGiovanni RA,Parcell AC,Winder WW,Thomson DM||Acta physiologica (Oxford, England) (201:457)||2011|
Exercise and PGC-1¿-independent synchronization of type I muscle metabolism and vasculature by ERR¿.
PA1-055 was used in western blot to investigate the role of ERR gamma in coordinating type I muscle metabolism and vascular transformation in muscles
|Narkar VA,Fan W,Downes M,Yu RT,Jonker JW,Alaynick WA,Banayo E,Karunasiri MS,Lorca S,Evans RM||Cell metabolism (13:283)||2011|
Exercise training reverses impaired skeletal muscle metabolism induced by artificial selection for low aerobic capacity.
PA1-055 was used in western blot to investigate the effect of exercise on the treatment of muscle impairment
|Lessard SJ,Rivas DA,Stephenson EJ,Yaspelkis BB,Koch LG,Britton SL,Hawley JA||American journal of physiology. Regulatory, integrative and comparative physiology (300:R175)||2011|
GDP and carboxyatractylate inhibit 4-hydroxynonenal-activated proton conductance to differing degrees in mitochondria from skeletal muscle and heart.
PA1-055 was used in western blot to investigate the differences of proton conductance mechanism between skeletal muscle and heart
|Aguirre E,Cadenas S||Biochimica et biophysica acta (1797:1716)||2010|
Loss of bradykinin signaling does not accelerate the development of cardiac dysfunction in type 1 diabetic akita mice.
PA1-055 was used in western blot to investigate the involvement of bradykinin signaling in diabetic cardiomyopathy
|Wende AR,Soto J,Olsen CD,Pires KM,Schell JC,Larrieu-Lahargue F,Litwin SE,Kakoki M,Takahashi N,Smithies O,Abel ED||Endocrinology (151:3536)||2010|
Impaired skeletal muscle beta-adrenergic activation and lipolysis are associated with whole-body insulin resistance in rats bred for low intrinsic exercise capacity.
PA1-055 was used in western blot to investigate the effect of beta-adrenergic signal transduction on metabolic health
|Lessard SJ,Rivas DA,Chen ZP,van Denderen BJ,Watt MJ,Koch LG,Britton SL,Kemp BE,Hawley JA||Endocrinology (150:4883)||2009|
AMP-activated protein kinase response to contractions and treatment with the AMPK activator AICAR in young adult and old skeletal muscle.
PA1-055 was used in western blot to investigate the influence of AICAR or muscle contraction on AMPK activity in old and young adult rat muscles
|Thomson DM,Brown JD,Fillmore N,Ellsworth SK,Jacobs DL,Winder WW,Fick CA,Gordon SE||The Journal of physiology (587:2077)||2009|
c-Cbl-deficient mice have reduced adiposity, higher energy expenditure, and improved peripheral insulin action.
PA1-055 was used in western blot to study the role of casitas b-lineage lymphoma (c-Cbl) in the whole-body fuel homeostasis.
|Molero JC,Jensen TE,Withers PC,Couzens M,Herzog H,Thien CB,Langdon WY,Walder K,Murphy MA,Bowtell DD,James DE,Cooney GJ||The Journal of clinical investigation (114:1326)||2004|
Expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in developing rat heart: putative involvement in control of mitochondrial membrane potential.
PA1-055 was used in western blot to investigate the expression of mitochondrial uncoupling protein 3 and adenine nucleotide translocase 1 genes in rat heart during development and its role in mitochondrial membrane potential
|Skárka L,Bardová K,Brauner P,Flachs P,Jarkovská D,Kopecký J,Ostádal B||Journal of molecular and cellular cardiology (35:321)||2003|
Skeletal muscle dysfunction is associated with derangements in mitochondrial bioenergetics (but not UCP3) in a rodent model of sepsis.
PA1-055 was used in western blot to assess the metabolic, cardiovascular, and skeletal muscle contractile changes after induction of peritoneal sepsis in wild-type and Ucp3 knockout mice
|Zolfaghari PS,Carré JE,Parker N,Curtin NA,Duchen MR,Singer M||American journal of physiology. Endocrinology and metabolism (308:E713)||2015|