PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.
PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.
The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.
The uncoupling proteins (UCPs) are found in the inner mitochondrial membranes and belong to a family of mitochondrial anion transport proteins that include the phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP-1 is abundantly expressed in brown adipose tissue of mammals. UCP-1 allows proton re-entry into the mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is thought that UCP-2 and 3 also dissipate the mitochondrial proton gradient produced by the respiratory chain in cells and tissues within which they are expressed. Studies have shown UCP-2 to be expressed by a variety of tissues. UCP-3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in heart and smooth muscle, and is absent in liver and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Mitochondrial uncoupling protein 3; Mitochondrial Uncoupling Protein-3; Solute carrier family 25 member 9; UCP 3; UCP-3; UCP3; Uncoupling protein 3, mitochondrial
Gene Aliases: AI645527; SLC25A9; UCP-3; UCP3
Molecular Function: RNA binding protein amino acid transporter calcium-binding protein calmodulin intracellular calcium-sensing protein mitochondrial carrier protein nucleic acid binding ribosomal protein transfer/carrier protein transporter