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Invitrogen
UCP3 Polyclonal Antibody
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FIGURE: 1 / 12
UCP3 Antibody (PA1-055) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of L6 (Lane 1) and C2C12 (Lane 2). The blot was probed with Anti-UCP3 Rabbit Polyclonal Antibody (Product # PA1-055, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 34 kDa band corresponding to UCP3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® ; 12% Bis-... View More
Product Details
PA1-055
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UCP-3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-055 detects uncoupling protein-3 (UCP-3) mouse, rat, and canine tissues. This antibody will also detect recombinant human UCP-3, but does not detect UCP-3 from mouse liver or kidney.
PA1-055 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~30 kDa protein representing UCP-3 from rat heart homogenate.
The PA1-055 immunogen is a synthetic peptide corresponding to residues C R(295) A L M K V Q V L R E S P F(308) of mouse and rat UPC-3. This peptide differs from the porcine and human UCP-3 sequence by a single amino acid substitution. PA1-055 immunizing peptide (Cat. # PEP-074) is available for use in neutralization and control experiments.
Target Information
The uncoupling protein UCP1 (formerly designated UCP) is an integral membrane protein unique to brown adipose tissue mitochondria. UCP1 forms a dimer that acts as a proton channel, which can uncouple oxidative phosphorylation by dissipating the electrochemical potential across the inner mitochondrial membrane. This process induces heat production in brown adipose tissue and is involved in regulation of body temperature and glucose metabolism. UCP2 is a structurally related protein that also uncouples mitochondrial respiration. It is more widely expressed in human and mouse tissues, including white adipose tissue and muscle, than is UCP1. UCP2 is thought to play a role in body weight regulation. An additional UCP family member, UCP3, is highly muscle specific and is possibly involved in the uncoupling of oxidative phosphorylation in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: mitochondrial uncoupling protein 3; Putative mitochondrial transporter UCP3; Solute carrier family 25 member 9; UCP 3; uncoupling protein; Uncoupling protein 3; Uncoupling protein 3, mitochondrial; Uncoupling protein-3
Gene Aliases: AI645527; SLC25A9; UCP-3; UCP3
UniProt ID: (Dog) Q9N2I9, (Mouse) P56501, (Rat) P56499
Entrez Gene ID: (Dog) 403575, (Mouse) 22229, (Rat) 25708
hydrogen ion transmembrane transport
response to superoxide
response to hypoxia
fatty acid metabolic process
transport
mitochondrial transport
proton transport
transmembrane transport
aging
response to nutrient
response to cold
response to activity
response to insulin
cellular response to hormone stimulus
response to steroid hormone
response to glucocorticoid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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