PA1-24895 detects UCP3 from human, mouse, and rat samples. The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids respectively. This antibody exhibits no cross reactivity with UCP1 or UCP2.
PA1-24895 has been successfully used in Western blot and immunohistochemistry (paraffin) procedures.
The PA1-24895 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
The uncoupling proteins (UCPs) are found in the inner mitochodrial membranes and belong to a family of mitochondrial anion transport proteins that include phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissues of mammals. UCP 1 allows proton re-entry into mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is though that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by th e respiratory chaun in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in the heart and smooth muscle, and is absent in the liver and kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Mitochondrial uncoupling protein 3; SLC25A9; Solute carrier family 25 member 9; UCP 3; uncoupling protein 3 (mitochondrial, proton carrier); Uncoupling protein 3, mitochondrial
Gene Aliases: AI645527; SLC25A9; UCP-3; UCP3
Molecular Function: RNA binding protein amino acid transporter calcium-binding protein calmodulin intracellular calcium-sensing protein mitochondrial carrier protein nucleic acid binding ribosomal protein transfer/carrier protein transporter