|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:3000|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-24895 detects UCP3 from human, mouse, and rat samples. The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids respectively. This antibody exhibits no cross reactivity with UCP1 or UCP2.
PA1-24895 has been successfully used in Western blot and immunohistochemistry (paraffin) procedures.
The PA1-24895 immunogen is a synthetic peptide, conjugated to KLH, corresponding to residues 254-267 of human UCP3 with an N-terminal added lysine.
The uncoupling proteins (UCPs) are found in the inner mitochodrial membranes and belong to a family of mitochondrial anion transport proteins that include phosphate and oxologutarate carriers and the ADP/ATP translocator. Three UCP subtypes have been identified and cloned. UCP 1 is abundantly expressed in brown adipose tissues of mammals. UCP 1 allows proton re-entry into mitochondrial matrix without involving the F0-F1 ATPase which produces heat instead of ATP. It is though that UCP 2 and 3 also dissipate the mitochondrial proton gradient produced by th e respiratory chaun in cells and tissues within which they are expressed. Studies have shown UCP 2 to be expressed by a variety of tissues. UCP 3 is expressed predominantly in brown adipose tissue, skeletal muscle, at lower levels in the heart and smooth muscle, and is absent in the liver and kidneys.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mitochondrial dysfunction in breast cancer cells prevents tumor growth: understanding chemoprevention with metformin.
PA1-24895 was used in western blot to study the inhibition of tumor growth caused by mitochondrial dysfunction in the epithelial cancer cell compartment
|Sanchez-Alvarez R,Martinez-Outschoorn UE,Lamb R,Hulit J,Howell A,Gandara R,Sartini M,Rubin E,Lisanti MP,Sotgia F||Cell cycle (Georgetown, Tex.) (12:172)||2013|