Western blot analysis of UCP4 using A) 30 µg SK-N-SH whole cell lysate (B) 30 µg IMR32 whole cell lysate and C) 30 µg SK-N-AS whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a UCP4 polyclonal antibody (Product # PA5-34952) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 13 and 174 of UCP4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34952 targets UCP4 in WB applications and shows reactivity with Human samples.
The PA5-34952 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 13 and 174 of UCP4.
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. May play a role in thermoregulatory heat production and metabolism in brain.
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