Proteintech

UFC1 Polyclonal Antibody

View all (7) UFC1 antibodies

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Antibody Testing Data (8)

UFC1 Antibody in Western Blot (WB)

FIGURE: 1 / 8

UFC1 Antibody (15783-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 15783-1-AP) UFC1 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

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UFC1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 15783-1-AP) UFC1 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver tissue slide using (Product # 15783-1-AP) (UFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 15783-1-AP (UFC1 antibody) at dilution of 1:100 (under 40x lens).

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 15783-1-AP (UFC1 antibody) at dilution of 1:100 (under 10x lens).

UFC1 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UFC1 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UFC1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

UFC1 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

15783-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

UFC1 Fusion Protein Ag8480 (1-167 aa encoded by BC005187)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Product # 15783-1-AP specifically recognizes the 21 kDa protein.

Immunogen sequence: MADEATRRV VSEIPVLKTN AGPRDRELWV QRLKEEYQSL IRYVENNKNA DNDWFRLESN KEGTRWFGKC WYIHDLLKYE FDIEFDIPIT CPTTAPEIAV PELDGKTAKM YRGGKICLTD HFKPLWARNV PKFGLAHLMA LGLGPWLAVE IPDLIQKGVI QHKEKCNQ (1-167 aa encoded by BC005187)

Target Information

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (Komatsu et al., 2004 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.

The Journal of biological chemistry

Gavin JM,Hoar K,Xu Q,Ma J,Lin Y,Chen J,Chen W,Bruzzese FJ,Harrison S,Mallender WD,Bump NJ,Sintchak MD,Bence NF,Li P,Dick LR,Gould AE,Chen JJ

Fri Aug 15 00:00:00 EDT 2014

Mechanistic study of Uba5 enzyme and the Ufm1 conjugation pathway.

The Journal of biological chemistry

Gavin JM,Hoar K,Xu Q,Ma J,Lin Y,Chen J,Chen W,Bruzzese FJ,Harrison S,Mallender WD,Bump NJ,Sintchak MD,Bence NF,Li P,Dick LR,Gould AE,Chen JJ

Fri Aug 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1; Ufm1-conjugating enzyme 1-

Gene Aliases: 1110021H02Rik; AA062310; CGI-126; ESTM29; HSPC155; UFC1; ufc1-s

UniProt ID: (Human) Q9Y3C8, (Rat) Q6BBI8, (Mouse) Q9CR09

Entrez Gene ID: (Human) 51506, (Rat) 445268, (Mouse) 66155

response to endoplasmic reticulum stress protein ufmylation protein polyufmylation protein K69-linked ufmylation

Process(es)

protein binding UFM1 transferase activity