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UFM1 Antibody (PA5-90754) in WB
Western blot analysis of UFM1 in extracts of various cell lines. Samples were incubated with UFM1 Polyclonal antibody (Product # PA5-90754) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-90754
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human UFM1
Conjugate
Unconjugated
Form
Liquid
Concentration
0.85 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2806222
Product Specific Information
Immunogen sequence: MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTA
Positive Samples: Mouse pancreas; Cellular Location: Cytoplasm, Nucleus
Target Information
UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al, 2004).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bA131P10.1; Ubiquitin-fold modifier 1
Gene Aliases: 1810045K17Rik; AI132708; AI463323; BM-002; C13orf20; ENSMUSG00000074598; Gm10726; RGD1304890; UFM1
UniProt ID: (Human) P61960, (Mouse) P61961, (Rat) Q5BJP3
Entrez Gene ID: (Human) 51569, (Mouse) 67890, (Rat) 365797
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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