Invitrogen

UFM1 Polyclonal Antibody

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UFM1 Antibody in Western Blot (WB) — Western Blot analysis of UFM1 in extracts of mouse pancreas using UFM1 Polyclonal Antibody (Product # PA5-90754) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-90754

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human UFM1 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	2.04 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2806222

Product Specific Information

Immunogen sequence: MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTAGNVFLKH GSELRIIPRD RVGSC; Positive Samples: Mouse pancreas; Cellular Location: Cytoplasm, Nucleus

Target Information

UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1; MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M; MIM 603173) (Komatsu et al, 2004).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.