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Invitrogen
UGT1A1 Polyclonal Antibody
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FIGURE: 1 / 5
UGT1A1 Antibody (PA5-80204) in IHC (P)
Immunohistochemistry analysis of UGT1A1 on paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with UGT1A1 polyclonal antibody (Product# PA5-80204) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°... View More
Product Details
PA5-80204
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human UGT1A1 recombinant protein (Position: E316-H533).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue. IHC: rat small intestine tissue, human rectal cancer tissue, mouse small intestine tissue, human mammary cancer tissue.
Target Information
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B1; bilirubin UDP-glucuronosyltranserase; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; Bilirubin-specific UDPGT isozyme 1; EC 2.4.1.17; HUG BR1; hUG-BR1; HUGBR1; UDP glucuronosyltransferase 1 family, polypeptide A1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1 family member 1; UDP-glucuronosyltransferase 1 family, member 1; UDP-glucuronosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A; UDP-glucuronosyltransferase 1A isoform 1; UDP-glucuronosyltransferase 1A1; UDPGT; UDPGT 1-1; UGT 1A; UGT-1A; UGT1 01; UGT1*1; UGT1-01; UGT1.1; UGT1A1; UGTBR1; unnamed protein product
Gene Aliases: BILIQTL1; GNT1; HUG-BR1; UDPGT; UDPGT 1-1; Udpgt-1a; UGT1; UGT1A; UGT1A01; UGT1A1; UgtBr1
UniProt ID: (Human) P22309, (Mouse) Q63886, (Rat) Q64550
Entrez Gene ID: (Human) 54658, (Mouse) 394436, (Rat) 24861
retinoic acid binding
enzyme inhibitor activity
steroid binding
protein binding
UDP-glycosyltransferase activity
glucuronosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
enzyme binding
protein homodimerization activity
protein heterodimerization activity
transferase activity, transferring hexosyl groups
glycosyltransferase
lipid metabolic process
bilirubin conjugation
xenobiotic metabolic process
steroid metabolic process
estrogen metabolic process
flavonoid metabolic process
retinoic acid metabolic process
negative regulation of fatty acid metabolic process
negative regulation of steroid metabolic process
flavone metabolic process
metabolic process
flavonoid biosynthetic process
response to organic substance
cellular response to hormone stimulus
negative regulation of catalytic activity
cellular glucuronidation
flavonoid glucuronidation
xenobiotic glucuronidation
biphenyl catabolic process
negative regulation of cellular glucuronidation
liver development
acute-phase response
response to nutrient
response to organic cyclic compound
organ regeneration
response to lipopolysaccharide
response to drug
response to starvation
response to ethanol
response to steroid hormone
response to glucocorticoid
cellular response to ethanol
cellular response to glucocorticoid stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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