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Product Details
PA5-96723
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50 - 1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human UGT2B10. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.32 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2808525 |
Product Specific Information
Immunogen sequence: GSCGKVLVWA AEYSLWMNMK TILKELVQRG HEVTVLASSA SILFDPNDSS TLKLEVYPTS LTKTEFENII MQLVKRLSEI QKDTFWLPFS QEQEILWAIN DIIRNFCKDV VSNKKLMKKL QESRFDIVFA DAYLPCGELL AELFNIPFVY SHSFSPGYSF ERHSGGFIFP PSYVPVVMSK LSDQMTFMER VKNMLYVLYF DFWFQIFNMK KWDQFYSEVL GRPTTLSETM RKADIWLMRN SWNFKFPHPF LPNVDFVGGL H; Positive Samples: 293T, SKOV3, U-937, Mouse liver, Mouse kidney, Rat liver; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
Target Information
DNA containing double-strand breaks (DSB) created by the CRISPR/Cas9 system can be repaired by either the non-homologous end-joining (NHEJ) or the homology-directed repair (HDR) pathway. The NHEJ repair pathway introduces non-specific insertions or deletions at the cleavage site, whereas the HDR pathway allows for precise gene editing at the DSB site. Target-specific HDR Plasmids provide a DNA repair template for a DSB and, when co-transfected with CRISPR/Cas9 KO Plasmids, enable the insertion of specific selection markers where Cas9-induced DNA cleavage has occurred. The HDR plasmid can incorporate a Red Fluorescent Protein (RFP) gene to visually confirm transfection and an antibiotic resistance gene (puromycin) for selection of cells containing a successful CRISPR/Cas9 doublestrand break. The puromycin resistance and RFP encoding genes are flanked by two LoxP sites that are recognized by the Cre Vector, which can be used to later remove these selection markers from the genomic DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: UDP glucuronosyltransferase 2 family, polypeptide B10; UDP glucuronosyltransferase 2 family, polypeptide B34; UDP glycosyltransferase 2 family, polypeptide B10; UDP-glucuronosyltransferase 2B10; UDPGT 2B10
Gene Aliases: UDPGT2B10; UGT2B10; Ugt2b34
UniProt ID: (Human) P36537
Entrez Gene ID: (Human) 7365, (Rat) 305264
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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