|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 51 and 516 of Human UHRF1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-27969 targets UHRF1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27969 immunogen is recombinant fragment corresponding to a region within amino acids 51 and 516 of Human UHRF1.
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex.
PA5-27969 was used in western blot to study the relationship between nuclear LASP-1 and the epigenetic machinery in breast cancer
|Duvall-Noelle N,Karwandyar A,Richmond A,Raman D||Oncogene (35:1122)||2016|