Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of UHRF1 showing staining in the nucleus of Hela cells using a UHRF1 polyclonal antibody (Product # PA5-35367) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 229-257 amino acids from the central region of human UHRF1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
UHRF1 is a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone acetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ICBP90; inverted CCAAT box-binding protein of 90 kDa; NP95; nuclear phosphoprotein 95; nuclear protein 95; nuclear zinc finger protein Np95; RING finger protein 106; RNF106; transcription factor ICBP90; Ubiquitin-like PHD and RING finger do; ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; UHRF1
hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1