Invitrogen

UNC84B Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (8) UNC84B antibodies

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Datasheet

Protocols

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UNC84B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of UNC84B was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with UNC84B Rabbit Polyclonal Antibody (Product # PA5-51539) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear and Nuclear membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

UNC84B Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of UNC84B in human cerebral cortex using an UNC84B Polyclonal Antibody (Product # PA5-51539) shows moderate to strong positivity in nuclear membrane in neuronal cells and glial cells.

UNC84B Antibody in Western Blot (WB) — Western blot was performed using Anti-UNC84B Polyclonal Antibody (Product # PA5-51539) and a 80 kDa band corresponding to UNC84B was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), Jurkat (Lane 4) and A549 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

UNC84B Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with, control siRNA, UNC84B siRNA probe #1, and a decrease in signal intensity was observed in Western Blot application using Anti-UNC84B Polyclonal Antibody (Product # PA5-51539) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Product Details

PA5-51539

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human UNC84B View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2649276

Product Specific Information

Immunogen sequence: LKSEWQSMTQ ESFQESSVKE LRRLEDQLAG LQQELAALAL KQSSVAEEVG LLPQQIQAVR DDVESQFPAW ISQFLARGGG GRVGLLQREE MQAQLRELES KILTHVAEMQ GKSAREAAAS LSLTLQKEGV IGVTEEQVHH IVKQALQRYS E

Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 80%.

Target Information

UNC84B may form a physical interaction between the nuclear envelope and the centrosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Nature

Procter DJ,Furey C,Garza-Gongora AG,Kosak ST,Walsh D

PA5-51539 was used in Immunocytochemistry to demonstrate the extent to which viruses can control the nucleus from the cytoplasm.

Sun Nov 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: nuclear envelope protein; Protein unc-84 homolog B; Rab5-interacting protein; Rab5IP; Sad1 unc-84 domain protein 2; Sad1/unc-84 protein-like 2; SUN domain-containing protein 2; unc-84 homolog B

Gene Aliases: FRIGG; KIAA0668; RAB5IP; SUN2; UNC84B

UniProt ID: (Human) Q9UH99

Entrez Gene ID: (Human) 25777

Molecular Function: cytoskeletal protein microtubule or microtubule-binding cytoskeletal protein non-motor microtubule binding protein

Function(s)

nuclear envelope organization mitotic spindle organization nuclear migration positive regulation of cell migration nuclear migration along microfilament centrosome localization cytoskeletal anchoring at nuclear membrane nuclear matrix anchoring at nuclear membrane

Process(es)

protein binding lamin binding microtubule binding identical protein binding

UNC84B Polyclonal Antibody

Product Details

PA5-51539

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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