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Invitrogen
UNG Polyclonal Antibody
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FIGURE: 1 / 4
UNG Antibody (PA5-20264) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human heart tissue using UNG Polyclonal Antibody (Product # PA5-20264) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Cou... View More
Product Details
PA5-20264
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide corresponding to 13 amino acids near the carboxy terminus of human UNG1. The immunogen is located within the last 50 amino acids of UNG1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is C2C12 cell lysate.
PA5-20264 can be used with blocking peptide PEP-0379.
Target Information
The human UNG gene encodes both nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase. These forms are generated by alternative splicing and the use of two differentially regulated promoters, PA and PB. The cDNAs for UNG1 and UNG2 are of similar size (2061 and 2058 bp, respectively) and as a result the corresponding mRNAs are not resolved as two species in gel electrophoresis. Nuclear UNG2 differs from mitochondrial UNG1 in 44 amino acids of the N-terminal sequence that is not necessary for catalytic activity. A major role of the UNG gene products is to repair mutagenic U:G mispairs caused by cytosine deamination. For example, UNG2 removes misincorporated dUMP residues. The level and expression pattern of UNG1 and UNG2 differs between cell and tissue type. Additionally, the expression of UNG is cell cycle regulated. The expression and activity of UNG has been found in general to be higher in proliferating as compared to nonproliferating tissues and cells. UNG1 is a 304 amino acid protein. UNG2 is a 313 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781L1143; UDG; UNG; Uracil-DNA glycosylase; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; uracil-DNA glycosylase nuclear isoform
Gene Aliases: DGU; HIGM4; HIGM5; UDG; UNG; UNG1; UNG15; UNG2
UniProt ID: (Human) P13051, (Mouse) P97931
Entrez Gene ID: (Human) 7374, (Mouse) 22256
damaged DNA binding
uracil DNA N-glycosylase activity
protein binding
enzyme binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing N-glycosyl compounds
uridine nucleosidase activity
DNA metabolism protein
DNA glycosylase
nucleic acid metabolism protein
DNA repair
base-excision repair
viral process
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
negative regulation of apoptotic process
depyrimidination
positive regulation of isotype switching
base-excision repair, AP site formation via deaminated base removal
cellular response to DNA damage stimulus
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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