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Invitrogen
UPF1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
UPF1 Antibody (PA5-54031) in ICC/IF
Immunofluorescence analysis of UPF1 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with UPF1 Polyclonal Antibody (Product # PA5-54031) at 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:20... View More
Product Details
PA5-54031
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human UPF1. Recombinant protein control fragment (Product #RP-91824).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2649284
Product Specific Information
Immunogen sequence: LEELWKENPS ATLEDLEKPG VDEEPQHVLL RYEDAYQYQN IFGPLVKLEA DYDKKLKESQ TQDNITVRWD LGLNKKRIAY FTLPKTD
Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
Target Information
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons. Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm and was later shown to be a component of cytoplasmic P-bodies. Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery. Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase RENT1; delta helicase; FLJ43809; FLJ46894; hUpf1; mUpf1; Nonsense mRNA reducing factor 1; NORF1; Regulator of nonsense transcripts 1; Regulator of nonsense transcripts 1 (Nonsense mRNA reducing factor 1) (NORF1) (Up-frameshift suppressor 1 homolog); smg-2 homolog, nonsense mediated mRNA decay factor; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1 regulator of nonsense transcripts homolog; UPF1 regulator of nonsense transcripts homolog (yeast); yeast Upf1p homolog
Gene Aliases: B430202H16Rik; HUPF1; KIAA0221; NORF1; PNORF-1; pNORF1; RENT1; smg-2; UPF1; Upflp
UniProt ID: (Human) Q92900, (Mouse) Q9EPU0
Entrez Gene ID: (Human) 5976, (Rat) 684558, (Mouse) 19704
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
nuclear-transcribed mRNA catabolic process
DNA replication
DNA repair
regulation of translational termination
dosage compensation by inactivation of X chromosome
histone mRNA catabolic process
nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay
telomere maintenance
mRNA export from nucleus
telomere maintenance via semi-conservative replication
positive regulation of mRNA catabolic process
3'-UTR-mediated mRNA destabilization
cellular response to lipopolysaccharide
cellular response to interleukin-1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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