Invitrogen

UQCRC2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (16) UQCRC2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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UQCRC2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of UQCRC2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with UQCRC2 Rabbit Polyclonal Antibody (Product # PA5-30204) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

UQCRC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of UQCRC2 in the cytoplasm of paraffin-embedded breast carcinoma using a UQCRC2 polyclonal antibody (Product # PA5-30204) at a dilution of 1:250.

UQCRC2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HepG2 (Lane 1), CaCo2 (Lane 2), A-431 (Lane 3), Jurkat (Lane 4), U937 untreated (Lane 5) or treated with LPS (100 ng/mL for 24 hours) (Lane 6). The blot was probed with Anti-UQCRC2 Polyclonal Antibody (Product # PA5-30204, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to UQCRC2 was observed in all the cell lines tested and was observed to be induced upon LPS treatment of U937 cells.

UQCRC2 Antibody in Immunoprecipitation (IP) — PA5-30204 immunoprecipitates UQCRC2 protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of UQCRC2 protein by 4 µg UQCRC2 antibody (Product # PA5-30204) on 10% SDS-PAGE.

UQCRC2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using UQCRC2 Polyclonal Antibody (Product # PA5-30204), shows increased expression of proteins in U937 cell line upon LPS treatment. {TM}

Product Details

PA5-30204

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:20,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Rat, Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 207 and 453 of Human UQCRC2 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547678

Product Specific Information

Recommended positive controls: Jurkat, Raji, NCI-H929, mouse brain, rat brain.

Predicted reactivity: Mouse (82%), Rat (81%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Absence of TXNIP in Humans Leads to Lactic Acidosis and Low Serum Methionine Linked to Deficient Respiration on Pyruvate.

Diabetes

Katsu-Jiménez Y,Vázquez-Calvo C,Maffezzini C,Halldin M,Peng X,Freyer C,Wredenberg A,Giménez-Cassina A,Wedell A,Arnér ESJ

PA5-30204 was used in Western Blotting to study a family with a mutation in the TXNIP gene leading to nondetectable expression of TXNIP protein.

Mon Apr 01 00:00:00 EDT 2019

Nuclear respiratory factor-1 and bioenergetics in tamoxifen-resistant breast cancer cells.

Experimental cell research

Radde BN,Ivanova MM,Mai HX,Alizadeh-Rad N,Piell K,Van Hoose P,Cole MP,Muluhngwi P,Kalbfleisch TS,Rouchka EC,Hill BG,Klinge CM

Published figure using UQCRC2 polyclonal antibody (Product # PA5-30204) in Western Blot

Sat Sep 10 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Complex III subunit 2; Core protein II; Cytochrome b-c1 complex subunit 2, mitochondrial; cytochrome bc-1 complex core protein II; U MC3DN5; ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (Complex III subunit II); ubiquinol-cytochrome c reductase core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2

Gene Aliases: 1500004O06Rik; MC3DN5; QCR2; UQCR2; UQCRC2

UniProt ID: (Human) P22695, (Mouse) Q9DB77, (Rat) P32551

Entrez Gene ID: (Human) 7385, (Mouse) 67003, (Rat) 293448

Molecular Function: metalloprotease