Invitrogen

UQCRC2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View Details

View all (14) UQCRC2 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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UQCRC2 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of UQCRC2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with UQCRC2 Rabbit Polyclonal Antibody (Product # PA5-30204) at 5 µg/ml in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

UQCRC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of UQCRC2 in the cytoplasm of paraffin-embedded breast carcinoma using a UQCRC2 polyclonal antibody (Product # PA5-30204) at a dilution of 1:250.

UQCRC2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HepG2 (Lane 1), CaCo2 (Lane 2), A-431 (Lane 3), Jurkat (Lane 4), U937 untreated (Lane 5) or treated with LPS (100 ng/mL for 24 hours) (Lane 6). The blot was probed with Anti-UQCRC2 Polyclonal Antibody (Product # PA5-30204, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to UQCRC2 was observed in all the cell lines tested and was observed to be induced upon LPS treatment of U937 cells.

UQCRC2 Antibody in Immunoprecipitation (IP) — PA5-30204 immunoprecipitates UQCRC2 protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of UQCRC2 protein by 4 µg UQCRC2 antibody (Product # PA5-30204) on 10% SDS-PAGE.

UQCRC2 Antibody in Cell treatment — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using UQCRC2 Polyclonal Antibody (Product # PA5-30204), shows increased expression of proteins in U937 cell line upon LPS treatment. {TM}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1,000

Immunofluorescence (IF)

1:100-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:5,000-1:20,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 207 and 453 of Human UQCRC2

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547678

Product Specific Information

Recommended positive controls: Jurkat, Raji, NCI-H929, mouse brain, rat brain.

Predicted reactivity: Mouse (82%), Rat (81%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: Complex III subunit 2; Core protein II; Cytochrome b-c1 complex subunit 2, mitochondrial; cytochrome bc-1 complex core protein II; U MC3DN5; ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (Complex III subunit II); ubiquinol-cytochrome c reductase core protein II; Ubiquinol-cytochrome-c reductase complex core protein 2

Gene Aliases: 1500004O06Rik; MC3DN5; QCR2; UQCR2; UQCRC2

UniProt ID: (Human) P22695, (Mouse) Q9DB77, (Rat) P32551

Entrez Gene ID: (Human) 7385, (Mouse) 67003, (Rat) 293448

Molecular Function: hydrolase metalloprotease protease