Proteintech

USP11 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

USP11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HEK-293 cells using 22340-1-AP (USP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

USP11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using 22340-1-AP ( USP11 Antibody) at dilution of 1:50 (under 40x lens).

USP11 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

USP11 Antibody in Immunoprecipitation (IP) — IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug.

Product Details

22340-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP11 Fusion Protein Ag17829 (441-743 aa encoded by BC000350)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: YLSVPLLIS HKRVLEVFFI PMDPRRKPEQ HRLVVPKKGK ISDLCVALSK HTGISPERMM VADVFSHRFY KLYQLEEPLS SILDRDDIFV YEVSGRIEAI EGSREDIVVP VYLRERTPAR DYNNSYYGLM LFGHPLLVSV PRDRFTWEGL YNVLMYRLSR YVTKPNSDDE DDGDEKEDDE EDKDDVPGPS TGGSLRDPEP EQAGPSSGVT NRCPFLLDNC LGTSQWPPRR RRKQLFTLQT VNSNGTSDRT TSPEEVHAQP YIAIDWEPEM KKRYYDEVEA EGYVKHDCVG YVMKKAPVRL QECI (441-743 aa encoded by B C000350)

Target Information

USP11 (ubiquitin specific peptidase 11), also known as UHX1, is a 920 amino acid deubiquitinating enzyme that participates in the Ub pathway. Localized to the nucleus, USP11 associates with both Ran BP-M (Ran binding protein M) and with the tumor suppressor BRCA2. Through these associations, USP11 functions to either inhibit ubiquitination of these proteins or to remove ubiquitin residues that have already been attached to these proteins. USP11 is implicated in several X-linked retinal diseases and, due to its ability to deubiquitinate BRCA2, may play a role in tumor suppression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia.

Science advances

Jin Q,Gutierrez Diaz B,Pieters T,Zhou Y,Narang S,Fijalkwoski I,Borin C,Van Laere J,Payton M,Cho BK,Han C,Sun L,Serafin V,Yacu G,Von Loocke W,Basso G,Veltri G,Dreveny I,Ben-Sahra I,Goo YA,Safgren SL,Tsai YC,Bornhauser B,Suraneni PK,Gaspar-Maia A,Kandela I,Van Vlierberghe P,Crispino JD,Tsirigos A,Ntziachristos P

Fri Dec 09 00:00:00 EST 2022

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women.

Cell

Yan Y,Wang X,Chaput D,Shin MK,Koh Y,Gan L,Pieper AA,Woo JA,Kang DE

Thu Oct 13 00:00:00 EDT 2022

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women.

Cell

Yan Y,Wang X,Chaput D,Shin MK,Koh Y,Gan L,Pieper AA,Woo JA,Kang DE

Thu Oct 13 00:00:00 EDT 2022

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women.

Cell

Yan Y,Wang X,Chaput D,Shin MK,Koh Y,Gan L,Pieper AA,Woo JA,Kang DE

Thu Oct 13 00:00:00 EDT 2022

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women.

Cell

Yan Y,Wang X,Chaput D,Shin MK,Koh Y,Gan L,Pieper AA,Woo JA,Kang DE

Thu Oct 13 00:00:00 EDT 2022

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

Cell death discovery

Gao Y,Chen B,Wang R,Xu A,Wu L,Lu H,Zhao G

Mon Aug 01 00:00:00 EDT 2022

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

Cell death discovery

Gao Y,Chen B,Wang R,Xu A,Wu L,Lu H,Zhao G

Mon Aug 01 00:00:00 EDT 2022

TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.

Cell reports

Luo Q,Wu X,Nan Y,Chang W,Zhao P,Zhang Y,Su D,Liu Z

Tue Jan 07 00:00:00 EST 2020

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Zhang S,Xie C,Li H,Zhang K,Li J,Wang X,Yin Z

Sun Jul 01 00:00:00 EDT 2018

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Zhang S,Xie C,Li H,Zhang K,Li J,Wang X,Yin Z

Sun Jul 01 00:00:00 EDT 2018

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Zhang S,Xie C,Li H,Zhang K,Li J,Wang X,Yin Z

Sun Jul 01 00:00:00 EDT 2018

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Zhang S,Xie C,Li H,Zhang K,Li J,Wang X,Yin Z

Sun Jul 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 11; RP4-659F15.2; Ubiquitin carboxyl-terminal hydrolase 11; ubiquitin carboxyl-terminal hydrolase, X-linked; Ubiquitin thioesterase 11; ubiquitin thiolesterase 11; ubiquitin-specific processing protease 11; Ubiquitin-specific-processing protease 11

Gene Aliases: UHX1; USP11

UniProt ID: (Human) P51784

Entrez Gene ID: (Human) 8237

Molecular Function: cysteine protease

Function(s)

ubiquitin-dependent protein catabolic process protein deubiquitination

Process(es)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding

USP11 Polyclonal Antibody

Product Details

22340-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!