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Product Details
16840-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
USP13 Fusion Protein Ag10527 (515-863 aa encoded by BC016146)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.31 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Product Specific Information
Immunogen sequence: IAYELTRRE AEANRRPLPE LVRAKIPFSA CLQAFSEPEN VDDFWSSALQ AKSAGVKTSR FASFPEYLVV QIKKFTFGLD WVPKKFDVSI DMPDLLDINH LRARGLQPGE EELPDISPPI VIPDDSKDRL MNQLIDPSDI DESSVMQLAE MGFPLEACRK AVYFTGNMGA EVAFNWIIVH MEEPDFAEPL TMPGYGGAAS AGASVFGASG LDNQPPEEIV AIITSMGFQR NQAIQALRAT NNNLERALDW IFSHPEFEED SDFVIEMENN ANANIISEAK PEGPRVKDGS GTYELFAFIS HMGTSTMSGH YICHIKKEGR WVIYNDHKVC ASERPPKDLG YMYFYRRIPS (515-863 aa encoded by BC016146)
Target Information
Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin thioesterase 13; ubiquitin thiolesterase 13; Ubiquitin-specific-processing protease 13
Gene Aliases: 2700071E21Rik; AI848077; IsoT-3; ISOT3; USP13
UniProt ID: (Human) Q92995, (Mouse) Q5BKP2
Entrez Gene ID: (Human) 8975, (Mouse) 72607, (Rat) 310306
Molecular Function:
cysteine protease
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
autophagy
cell proliferation
regulation of autophagy
melanocyte differentiation
protein stabilization
protein K63-linked deubiquitination
positive regulation of ERAD pathway
maintenance of unfolded protein involved in ERAD pathway
proteolysis
protein deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
zinc ion binding
ubiquitin protein ligase binding
ubiquitin binding
ubiquitin-like protein ligase binding
chaperone binding
proteasome binding
BAT3 complex binding
ubiquitin-specific protease activity involved in positive regulation of ERAD pathway
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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