Proteintech

USP14 Polyclonal Antibody

Product Image Gallery

USP14 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

USP14 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

USP14 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug USP14 antibody (14517-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

USP14 Antibody in Immunoprecipitation (IP) — IP result of anti-USP14 (IP:14517-1-AP, 4ug; Detection:14517-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (IHC)

Assay-dependent

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Published Applications

Immunohistochemistry (IHC)

See 2 publications below

Western Blot (WB)

See 2 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP14 Fusion Protein Ag5979

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ataxia; Deubiquitinating enzyme 14; dUB-type TGT for deubiquitinating enzyme; TGT; tRNA-guanine transglycosylase, 60-kD subunit; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin thioesterase 14; Ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14; USP14

Gene Aliases: 2610005K12Rik; 2610037B11Rik; AW107924; ax; C78769; nmf375; TGT; USP14

UniProt ID: (Human) P54578, (Mouse) Q9JMA1

Entrez Gene ID: (Human) 9097, (Mouse) 59025, (Rat) 291796

Function(s)

proteolysis ubiquitin-dependent protein catabolic process chemical synaptic transmission negative regulation of endopeptidase activity protein deubiquitination proteasome-mediated ubiquitin-dependent protein catabolic process regulation of chemotaxis regulation of proteasomal protein catabolic process negative regulation of ER-associated ubiquitin-dependent protein catabolic process

Process(es)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity endopeptidase inhibitor activity protein binding tRNA guanylyltransferase activity proteasome binding peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity