Proteintech

USP14 Polyclonal Antibody

Product Image Gallery

USP14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

USP14 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

USP14 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug USP14 antibody (14517-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

USP14 Antibody in Immunoprecipitation (IP) — IP result of anti-USP14 (IP:14517-1-AP, 4ug; Detection:14517-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

14517-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	Assay-dependent	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP14 Fusion Protein Ag5979 (1-294 aa encoded by BC003556) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MPLYSVTVK WGKEKFEGVE LNTDEPPMVF KAQLFALTGV QPARQKVMVK GGTLKDDDWG NIKIKNGMTL LMMGSADALP EEPSAKTVFV EDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRL (1-294 aa encoded by BC003556)

Target Information

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP14 as a novel prognostic marker promotes cisplatin resistance via Akt/ERK signaling pathways in gastric cancer.

Cancer medicine

Fu Y,Ma G,Liu G,Li B,Li H,Hao X,Liu L

Thu Nov 01 00:00:00 EDT 2018

USP14 as a novel prognostic marker promotes cisplatin resistance via Akt/ERK signaling pathways in gastric cancer.

Cancer medicine

Fu Y,Ma G,Liu G,Li B,Li H,Hao X,Liu L

Thu Nov 01 00:00:00 EDT 2018

Overexpression of ubiquitin specific peptidase 14 predicts unfavorable prognosis in esophageal squamous cell carcinoma.

Thoracic cancer

Zhang B,Li M,Huang P,Guan XY,Zhu YH

Sat Jul 01 00:00:00 EDT 2017

Overexpression of ubiquitin specific peptidase 14 predicts unfavorable prognosis in esophageal squamous cell carcinoma.

Thoracic cancer

Zhang B,Li M,Huang P,Guan XY,Zhu YH

Sat Jul 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: ataxia; Deubiquitinating enzyme 14; dUB-type TGT for deubiquitinating enzyme; tRNA-guanine transglycosylase, 60-kD subunit; Ubiquitin carboxyl-terminal hydrolase 14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14; ubiquitin specific protease 14 (tRNA-guanine transglycosylase); Ubiquitin thioesterase 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; Ubiquitin-specific-processing protease 14

Gene Aliases: 2610005K12Rik; 2610037B11Rik; AW107924; ax; C78769; nmf375; TGT; USP14

UniProt ID: (Human) P54578, (Mouse) Q9JMA1

Entrez Gene ID: (Human) 9097, (Mouse) 59025, (Rat) 291796

Molecular Function: cysteine protease protease protein modifying enzyme