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USP15 Polyclonal Antibody

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Antibody Testing Data (10)

USP15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

USP15 Antibody (14354-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue slide using (Product # 14354-1-AP) USP15 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

USP15 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with (Product # 14354-1-AP) USP15 antibody at dilution of 1:4,000 incubated at room temperature for 1.5 hours.

USP15 Antibody in Immunoprecipitation (IP) — IP result of anti-USP15 (IP:14354-1-AP, 4ug; Detection:14354-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

14354-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP15 Fusion Protein Ag5703 (1-235 aa encoded by BC020688)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.44 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MAEGGAADL DTQRSDIATL LKTSLRKGDT WYLVDSRWFK QWKKYVGFDS WDKYQMGDQN VYPGPIDNSG LLKDGDAQSL KEHLIDELDY ILLPTEGWNK LVSWYTLMEG QEPIARKVVE QGMFVKHCKV EVYLTELKLC ENGNMNNVVT RRFSKADTID TIEKEIRKIF SIPDEKETRL WNKYMSNTFE PLNKPDSTIQ DAGLYQGQVL VIEQKNEDGT WPRGPSTPKK PLEQSC (1-235 aa encoded by B C020688)

Target Information

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 EDT 2023

USP8 inhibition regulates autophagy flux and controls Salmonella infection.

Frontiers in cellular and infection microbiology

Santelices J,Ou M,Maegawa GHB,Hercik K,Edelmann MJ

Mon Apr 10 00:00:00 EDT 2023

USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion.

Cell death & disease

Tian X,Zhao Y,Yang Z,Lu Q,Zhou L,Zheng S

Mon Sep 26 00:00:00 EDT 2022

USP15 regulates p66Shc stability associated with Drp1 activation in liver ischemia/reperfusion.

Cell death & disease

Tian X,Zhao Y,Yang Z,Lu Q,Zhou L,Zheng S

Mon Sep 26 00:00:00 EDT 2022

The deubiquitinase USP15 modulates cellular redox and is a therapeutic target in acute myeloid leukemia.

Leukemia

Niederkorn M,Ishikawa C,M Hueneman K,Bartram J,Stepanchick E,R Bennett J,E Culver-Cochran A,Bolanos LC,Uible E,Choi K,Wunderlich M,Perentesis JP,M Chlon T,Filippi MD,Starczynowski DT

Tue Feb 01 00:00:00 EST 2022

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 EDT 2021

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

The Science of the total environment

Zhang L,Gao X,Qin Z,Shi X,Xu K,Wang S,Tang M,Wang W,Gao S,Zuo L,Zhang L,Zhang W

Fri Aug 20 00:00:00 EDT 2021

TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis.

Cell reports

Niederkorn M,Hueneman K,Choi K,Varney ME,Romano L,Pujato MA,Greis KD,Inoue JI,Meetei R,Starczynowski DT

Tue Feb 25 00:00:00 EST 2020

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

Autophagy

Niu K,Fang H,Chen Z,Zhu Y,Tan Q,Wei D,Li Y,Balajee AS,Zhao Y

Wed Apr 01 00:00:00 EDT 2020

USP33 deubiquitinates PRKN/parkin and antagonizes its role in mitophagy.

Autophagy

Niu K,Fang H,Chen Z,Zhu Y,Tan Q,Wei D,Li Y,Balajee AS,Zhao Y

Wed Apr 01 00:00:00 EDT 2020

USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair.

Frontiers in cell and developmental biology

Zhao Y,Huang X,Zhang Z,Zhang Y,Zhang G,Zan T,Li Q

Mon Sep 28 00:00:00 EDT 2020

Inhibition of USP15 Prevent Glutamate-Induced Oxidative Damage by Activating Nrf2/HO-1 Signaling Pathway in HT22 Cells.

Cellular and molecular neurobiology

Chen X,Bao G,Liu F

Sat Aug 01 00:00:00 EDT 2020

Disruption of the Keap1/Nrf2-Antioxidant Response System After Chronic Doxorubicin Exposure In Vivo.

Cardiovascular toxicology

Nordgren KKS,Wallace KB

Tue Dec 01 00:00:00 EST 2020

LncRNA 2810403D21Rik/Mirf promotes ischemic myocardial injury by regulating autophagy through targeting Mir26a.

Autophagy

Liang H,Su X,Wu Q,Shan H,Lv L,Yu T,Zhao X,Sun J,Yang R,Zhang L,Yan H,Zhou Y,Li X,Du Z,Shan H

Mon Jun 01 00:00:00 EDT 2020

Overexpression of Ubiquitin-Specific Protease15 (USP15) Promotes Tumor Growth and Inhibits Apoptosis and Correlated With Poor Disease-Free Survival in Hepatocellular Carcinoma.

Technology in cancer research & treatment

Yao XQ,Li L,Piao LZ,Zhang GJ,Huang XZ,Wang Y,Liang ZL

Mon Nov 08 00:00:00 EST 2021

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 EDT 2019

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 EDT 2019

Ubiquitin-Specific Protease 15 Maintains Transforming Growth Factor-β Pathway Activity by Deubiquitinating Transforming Growth Factor-β Receptor I during Wound Healing.

The American journal of pathology

Zhao Y,Wang Z,Ho C,Zhang G,Li Q

Mon Jul 01 00:00:00 EDT 2019

Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue.

Analytical biochemistry

Nanduri B,Shack LA,Rai AN,Epperson WB,Baumgartner W,Schmidt TB,Edelmann MJ

Thu Dec 15 00:00:00 EST 2016

Use of focused ultrasonication in activity-based profiling of deubiquitinating enzymes in tissue.

Analytical biochemistry

Nanduri B,Shack LA,Rai AN,Epperson WB,Baumgartner W,Schmidt TB,Edelmann MJ

Thu Dec 15 00:00:00 EST 2016

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

Nature immunology

Zou Q,Jin J,Hu H,Li HS,Romano S,Xiao Y,Nakaya M,Zhou X,Cheng X,Yang P,Lozano G,Zhu C,Watowich SS,Ullrich SE,Sun SC

Sun Jun 01 00:00:00 EDT 2014

USP15 stabilizes MDM2 to mediate cancer-cell survival and inhibit antitumor T cell responses.

Nature immunology

Zou Q,Jin J,Hu H,Li HS,Romano S,Xiao Y,Nakaya M,Zhou X,Cheng X,Yang P,Lozano G,Zhu C,Watowich SS,Ullrich SE,Sun SC

Sun Jun 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 15; deubiquitinating enzyme Ubp109; granule cell antiserum positive 18; MGC131982; MGC149838; MGC74854; Ubiquitin carboxyl-terminal hydrolase 15; Ubiquitin carboxyl-terminal hydrolase of 109 kDa; ubiquitin specific protease 15; Ubiquitin thioesterase 15; ubiquitin thiolesterase 15; Ubiquitin-specific-processing protease 15; Unph-2; Unph4

Gene Aliases: 4921514G19Rik; AI327321; E430033I05Rik; Gcap18; KIAA0529; Ubp109; UNPH-2; UNPH4; USP15

UniProt ID: (Human) Q9Y4E8, (Mouse) Q8R5H1, (Rat) Q9R085

Entrez Gene ID: (Human) 9958, (Mouse) 14479, (Rat) 171329

Molecular Function: cysteine protease