Proteintech

USP20 Polyclonal Antibody

View all (9) USP20 antibodies

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USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using (Product # 17491-1-AP) (USP20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 40x lens).

USP20 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung using 17491-1-AP (USP20 antibody) at dilution of 1:50 (under 10x lens).

USP20 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

USP20 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 17491-1-AP (USP20 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

USP20 Antibody in Western Blot (WB) — WB result of USP20 antibody (17491-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP20 transfected HeLa cells.

USP20 Antibody in Immunoprecipitation (IP) — IP result of anti-USP20 (IP:17491-1-AP, 4ug; Detection:17491-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

17491-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

USP20 Fusion Protein Ag11590 (1-351 aa encoded by BC039593)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MGDSRDLCP HLDSIGEVTK EDLLLKSKGT CQSCGVTGPN LWACLQVACP YVGCGESFAD HSTIHAQAKK HNLTVNLTTF RLWCYACEKE VFLEQRLAAP LLGSSSKFSE QDSPPPSHPL KAVPIAVADE GESESEDDDL KPRGLTGMKN LGNSCYMNAA LQALSNCPPL TQFFLECGGL VRTDKKPALC KSYQKLVSEV WHKKRPSYVV PTSLSHGIKL VNPMFRGYAQ QDTQEFLRCL MDQLHEELKE PVVATVALTE ARDSDSSDTD EKREGDRSPS EDEFLSCDSS SDRGEGDGQG RGGGSSQAET ELLIPDEAGR AISEKERMKD RKFSWGQQRT NSEQVDEDAD VD (1-351 aa encoded by BC039593)

Target Information

SLAIN2 is closely related to SLAIN1, a novel stem cell protein that is expressed at the stem cell and epiblast stages. Unlike SLAIN1, SLAIN2 is widely expressed and is thought to play a role in regulating microtubule (MT) growth and organization. SLAIN2 binds to end-binding proteins (EBs), cytoplasmic linker proteins (CLIPs) such as CLIP170, CLIP-associated proteins, and interacts with the MT polymerase ch-TOG. Depletion of SLAIN2 using RNAi led to disorganization of the radial MT array, indicating its importance in the regulation of MT growth and organization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP20 is a predictor of poor prognosis in colorectal cancer and associated with lymph node metastasis, immune infiltration and chemotherapy resistance.

Frontiers in oncology

Jin R,Luo Z,Tao Q,Wang P,Cai X,Jiang L,Zeng C,Chen Y

Tue Mar 07 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice.

Frontiers in endocrinology

Zhang W,Shi B,Li S,Liu Z,Li S,Dong S,Cheng Y,Zhu J,Zhang G,Zhong M

Tue Jan 17 00:00:00 EST 2023

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Diabetes

Niu Y,Jiang H,Yin H,Wang F,Hu R,Hu X,Peng B,Shu Y,Li Z,Chen S,Guo F

Sun May 01 00:00:00 EDT 2022

The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein.

Biochemical and biophysical research communications

Feng J,Liu P,Li X,Zhang D,Lin H,Hou Z,Guo C,Niu Y,Dai B,Wang O,Qi M,Wang H,Zhou H

Sat Feb 19 00:00:00 EST 2022

The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein.

Biochemical and biophysical research communications

Feng J,Liu P,Li X,Zhang D,Lin H,Hou Z,Guo C,Niu Y,Dai B,Wang O,Qi M,Wang H,Zhou H

Sat Feb 19 00:00:00 EST 2022

The deubiquitinating enzyme USP20 regulates the stability of the MCL1 protein.

Biochemical and biophysical research communications

Feng J,Liu P,Li X,Zhang D,Lin H,Hou Z,Guo C,Niu Y,Dai B,Wang O,Qi M,Wang H,Zhou H

Sat Feb 19 00:00:00 EST 2022

USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells.

Molecular therapy oncolytics

Lu R,Wu G,Chen M,Ji D,Liu Y,Zhou GG,Fu W

Fri Dec 17 00:00:00 EST 2021

USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells.

Molecular therapy oncolytics

Lu R,Wu G,Chen M,Ji D,Liu Y,Zhou GG,Fu W

Fri Dec 17 00:00:00 EST 2021

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

The Journal of cell biology

Culver JA,Mariappan M

Mon May 03 00:00:00 EDT 2021

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

The Journal of cell biology

Culver JA,Mariappan M

Mon May 03 00:00:00 EDT 2021

Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.

Genes & development

Li W,Shen M,Jiang YZ,Zhang R,Zheng H,Wei Y,Shao ZM,Kang Y

Thu Oct 01 00:00:00 EDT 2020

Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.

Genes & development

Li W,Shen M,Jiang YZ,Zhang R,Zheng H,Wei Y,Shao ZM,Kang Y

Thu Oct 01 00:00:00 EDT 2020

USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.

Cell death and differentiation

Wu C,Luo K,Zhao F,Yin P,Song Y,Deng M,Huang J,Chen Y,Li L,Lee S,Kim J,Zhou Q,Tu X,Nowsheen S,Luo Q,Gao X,Lou Z,Liu Z,Yuan J

Thu Nov 01 00:00:00 EDT 2018

USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.

Cell death and differentiation

Wu C,Luo K,Zhao F,Yin P,Song Y,Deng M,Huang J,Chen Y,Li L,Lee S,Kim J,Zhou Q,Tu X,Nowsheen S,Luo Q,Gao X,Lou Z,Liu Z,Yuan J

Thu Nov 01 00:00:00 EDT 2018

USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.

Cell death and differentiation

Wu C,Luo K,Zhao F,Yin P,Song Y,Deng M,Huang J,Chen Y,Li L,Lee S,Kim J,Zhou Q,Tu X,Nowsheen S,Luo Q,Gao X,Lou Z,Liu Z,Yuan J

Thu Nov 01 00:00:00 EDT 2018

USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.

Cell death and differentiation

Wu C,Luo K,Zhao F,Yin P,Song Y,Deng M,Huang J,Chen Y,Li L,Lee S,Kim J,Zhou Q,Tu X,Nowsheen S,Luo Q,Gao X,Lou Z,Liu Z,Yuan J

Thu Nov 01 00:00:00 EDT 2018

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

International journal of oncology

Wang C,Yang C,Ji J,Jiang J,Shi M,Cai Q,Yu Y,Zhu Z,Zhang J

Sat Apr 01 00:00:00 EDT 2017

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

International journal of oncology

Wang C,Yang C,Ji J,Jiang J,Shi M,Cai Q,Yu Y,Zhu Z,Zhang J

Sat Apr 01 00:00:00 EDT 2017

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

International journal of oncology

Wang C,Yang C,Ji J,Jiang J,Shi M,Cai Q,Yu Y,Zhu Z,Zhang J

Sat Apr 01 00:00:00 EDT 2017

Deubiquitinating enzyme USP20 is a positive regulator of Claspin and suppresses the malignant characteristics of gastric cancer cells.

International journal of oncology

Wang C,Yang C,Ji J,Jiang J,Shi M,Cai Q,Yu Y,Zhu Z,Zhang J

Sat Apr 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 20; hVDU2; pVHL-interacting deubiquitinating enzyme 2; Ubiquitin carboxyl-terminal hydrolase 20; ubiquitin specific protease 20; Ubiquitin thioesterase 20; ubiquitin thiolesterase 20; Ubiquitin-specific-processing protease 20; VHL-interacting deubiquitinating enzyme 2

Gene Aliases: 1700055M05Rik; AI467231; hVDU2; KIAA1003; LSFR3A; USP20; VDU2

UniProt ID: (Human) Q9Y2K6, (Mouse) Q8C6M1

Entrez Gene ID: (Human) 10868, (Mouse) 74270, (Rat) 311856

Molecular Function: cysteine protease

ubiquitin-dependent protein catabolic process endocytosis regulation of G-protein coupled receptor protein signaling pathway protein deubiquitination protein K63-linked deubiquitination protein K48-linked deubiquitination proteolysis proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal protein catabolic process

Process(es)

G-protein coupled receptor binding cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding zinc ion binding peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity metal ion binding