In IHC applications, antigen retrieval using a citrate buffer (pH 6.0) is recommended.
Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Monkey (93%) Orangutan, Gibbon, Marmoset, Dog, Bovine, Panda (86%).
DNA-directed RNA polymerase I subunit RPA43 or Twist neighbor protein (Twistnb)belongs to the eukaryotic RPA43 RNA polymerase subunit family. It catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.Twistnb is the component of RNA polymerase I which synthesizes ribosomal RNA precursors. It is widely expressed in all fetal and adult tissues, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues. Twist genes are essential for embryonic development and are conserved from jellyfish to human.
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Protein Aliases: Deubiquitinating enzyme 33; hVDU1; pVHL-interacting deubiquitinating enzyme 1; Ubiquitin carboxyl-terminal hydrolase 33; Ubiquitin thioesterase 33; ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; VHL-interacting deubiquitinating enzyme 1
Gene Aliases: KIAA1097; USP33; VDU1
UniProt ID: (Human) Q8TEY7
Entrez Gene ID: (Human) 23032