|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues in human USP35|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 113 kDa.
Purity is >95% by SDS-PAGE.
The ubiquitin pathway involves three sequential enzymatic steps that facilitate the conjugation of ubiquitin and ubiquitin-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the ubiquitin pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP35 (ubiquitin specific peptidase 35) is a 1,017 amino acid protein that belongs to the peptidase C19 family. Expressed in pancreas, testis and skeletal muscle, USP35 functions to catalyze the conversion of a ubiquitin C-terminal thioester and water to free ubiquitin and a thiol, a reaction that may play a role in signaling events throughout the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.