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Invitrogen
USP37 Polyclonal Antibody
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FIGURE: 1 / 3
USP37 Antibody (PA5-100697) in ICC/IF
Immunocytochemistry analysis of USP37 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with USP37 polyclonal antibody (Product # PA5-100697) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100697
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human USP37(Accession Q86T82), corresponding to amino acid residues P760-E810.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total USP37.
Target Information
Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 37; tmp_locus_50; Ubiquitin carboxyl-terminal hydrolase 37; ubiquitin specific peptidase 37; ubiquitin specific peptidase 37-like; ubiquitin specific protease 37; Ubiquitin thioesterase 37; ubiquitin thiolesterase 37; Ubiquitin-specific-processing protease 37
Gene Aliases: KIAA1594; USP37
UniProt ID: (Human) Q86T82
Entrez Gene ID: (Human) 57695, (Rat) 100361658
G1/S transition of mitotic cell cycle
regulation of DNA replication
proteolysis
protein deubiquitination
regulation of protein stability
protein K11-linked deubiquitination
cell division
protein K48-linked deubiquitination
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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