Invitrogen

USP48 Polyclonal Antibody

Product Image Gallery

USP48 Antibody in Western Blot (WB) — Western blot analysis of USP48 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an USP48 Polyclonal Antibody (Product # PA5-56320).

USP48 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of USP48 in human lateral ventricle shows positivity in a subset of neurons as well as neuropil with a accentuation around blood vessels. Samples were probed using an USP48 Polyclonal Antibody (Product # PA5-56320).

USP48 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of USP48 in mouse visual cortex shows immunoreactivity in neurons and endothelial cells. Samples were probed using an USP48 Polyclonal Antibody (Product # PA5-56320).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human USP48

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2649359

Product Specific Information

Immunogen sequence: QEKPNTTVQV PAFLQELVDR DNSKFEEWCI EMAEMRKQSV DKGKAKHEEV KELYQRLPAG AEPYEFVSLE WLQKWLDEST PTKPIDNHAC LCSHDKLHPD KISIMKRISE YAADIFYSRY GGGPRLTVKA LCKECVV

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

Target Information

USP48 is a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 48; Synaptic ubiquitin-specific protease; synUSP; Ubiquitin carboxyl-terminal hydrolase 48; ubiquitin specific protease 31; ubiquitin specific protease 48; Ubiquitin thioesterase 48; ubiquitin thiolesterase 48; Ubiquitin-specific peptidase 48; Ubiquitin-specific protease 48; Ubiquitin-specific-processing protease 48

Gene Aliases: 2810449C13Rik; AI115503; BC021769; D330022K21Rik; Kiaa4202; RAP1GA1; synUSP; USP31; USP48

UniProt ID: (Human) Q86UV5, (Rat) Q76LT8, (Mouse) Q3V0C5

Entrez Gene ID: (Human) 84196, (Rat) 362636, (Mouse) 170707

Function(s)

ubiquitin-dependent protein catabolic process protein deubiquitination proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal protein catabolic process proteolysis

Process(es)

thiol-dependent ubiquitin-specific protease activity cysteine-type endopeptidase activity peptidase activity cysteine-type peptidase activity hydrolase activity thiol-dependent ubiquitinyl hydrolase activity