Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:2,000-1:12,000 | View 14 publications 14 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:400 | - |
Immunocytochemistry (ICC/IF) |
- | View 2 publications 2 publications |
ELISA (ELISA) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | View 2 publications 2 publications |
Miscellaneous PubMed (Misc) |
- | View 3 publications 3 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
USP5 Fusion Protein Ag0770 (1-300 aa encoded by BC004889) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.4 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MAELSEEAL LSVLPTIRVP KAGDRVHKDE CAFSFDTPES EGGLYICMNT FLGFGKQYVE RHFNKTGQRV YLHLRRTRRP KEEDPATGTG DPPRKKPTRL AIGVEGGFDL SEEKFELDED VKIVILPDYL EIARDGLGGL PDIVRDRVTS AVEALLSADS ASRKQEVQAW DGEVRQVSKH AFSLKQLDNP ARIPPCGWKC SKCDMRENLW LNLTDGSILC GRRYFDGSGG NNHAVEHYRE TGYPLAVKLG TITPDGADVY SYDEDDMVLD PSLAEHLSHF GIDMLKMQKT DKTMTELEID M (1-300 aa encoded by BC004889)
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; ubiquitin carboxy terminal hydrolase T; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5
Gene Aliases: AA407472; ISOT; ISOT-1; Ucht; USP5
UniProt ID: (Human) P45974, (Mouse) P56399
Entrez Gene ID: (Human) 8078, (Rat) 297593, (Mouse) 22225
Molecular Function:
cysteine protease
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