Proteintech

USP5 Polyclonal Antibody

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USP5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

USP5 Antibody in Western Blot (WB) — Human colon tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

USP5 Antibody in Immunoprecipitation (IP) — IP result of anti-USP5 (IP:10473-1-AP, 4ug; Detection:10473-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Product Details

10473-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:12,000	View 14 publications 14 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	USP5 Fusion Protein Ag0770 (1-300 aa encoded by BC004889) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAELSEEAL LSVLPTIRVP KAGDRVHKDE CAFSFDTPES EGGLYICMNT FLGFGKQYVE RHFNKTGQRV YLHLRRTRRP KEEDPATGTG DPPRKKPTRL AIGVEGGFDL SEEKFELDED VKIVILPDYL EIARDGLGGL PDIVRDRVTS AVEALLSADS ASRKQEVQAW DGEVRQVSKH AFSLKQLDNP ARIPPCGWKC SKCDMRENLW LNLTDGSILC GRRYFDGSGG NNHAVEHYRE TGYPLAVKLG TITPDGADVY SYDEDDMVLD PSLAEHLSHF GIDMLKMQKT DKTMTELEID M (1-300 aa encoded by BC004889)

Target Information

USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3.2-USP5 interactions in the pain pathway.

Molecular pain

Stemkowski PL,Garcia-Caballero A,Gadotti VM,M'Dahoma S,Chen L,Souza IA,Zamponi GW

Tue Sep 04 00:00:00 EDT 2018

Selective inhibition of Ca_V3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.

Science signaling

Joksimovic SL,Joksimovic SM,Tesic V,García-Caballero A,Feseha S,Zamponi GW,Jevtovic-Todorovic V,Todorovic SM

Tue Aug 28 00:00:00 EDT 2018

Selective inhibition of Ca_V3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.

Science signaling

Joksimovic SL,Joksimovic SM,Tesic V,García-Caballero A,Feseha S,Zamponi GW,Jevtovic-Todorovic V,Todorovic SM

Tue Aug 28 00:00:00 EDT 2018

Ubiquitin-specific peptidase 5 and ovarian tumor deubiquitinase 6A are differentially expressed in p53+/+ and p53-/- HCT116 cells.

International journal of oncology

Kim SY,Kwon SK,Lee SY,Baek KH

Tue May 01 00:00:00 EDT 2018

Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities.

Journal of cell science

Xie X,Matsumoto S,Endo A,Fukushima T,Kawahara H,Saeki Y,Komada M

Thu Apr 12 00:00:00 EDT 2018

Deubiquitylases USP5 and USP13 are recruited to and regulate heat-induced stress granules through their deubiquitylating activities.

Journal of cell science

Xie X,Matsumoto S,Endo A,Fukushima T,Kawahara H,Saeki Y,Komada M

Thu Apr 12 00:00:00 EDT 2018

Zinc deficiency promotes cystitis-related bladder pain by enhancing function and expression of Ca_v3.2 in mice.

Toxicology

Ozaki T,Matsuoka J,Tsubota M,Tomita S,Sekiguchi F,Minami T,Kawabata A

Mon Jan 15 00:00:00 EST 2018

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.

Biochemical and biophysical research communications

Li XY,Wu HY,Mao XF,Jiang LX,Wang YX

Sat Oct 07 00:00:00 EDT 2017

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.

Biochemical and biophysical research communications

Li XY,Wu HY,Mao XF,Jiang LX,Wang YX

Sat Oct 07 00:00:00 EDT 2017

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.

Biochemical and biophysical research communications

Li XY,Wu HY,Mao XF,Jiang LX,Wang YX

Sat Oct 07 00:00:00 EDT 2017

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.

Cell death & disease

Wang S,Juan J,Zhang Z,Du Y,Xu Y,Tong J,Cao B,Moran MF,Zeng Y,Mao X

Thu Sep 21 00:00:00 EDT 2017

High glucose induces N-linked glycosylation-mediated functional upregulation and overexpression of Ca_v3.2 T-type calcium channels in neuroendocrine-like differentiated human prostate cancer cells.

Journal of pharmacological sciences

Fukami K,Asano E,Ueda M,Sekiguchi F,Yoshida S,Kawabata A

Sun Jan 01 00:00:00 EST 2017

A cell-permeant peptide corresponding to the cUBP domain of USP5 reverses inflammatory and neuropathic pain.

Molecular pain

Garcia-Caballero A,Gadotti VM,Chen L,Zamponi GW

Mon Dec 26 00:00:00 EST 2016

Smurf1 represses TNF-α production through ubiquitination and destabilization of USP5.

Biochemical and biophysical research communications

Qian G,Ren Y,Zuo Y,Yuan Y,Zhao P,Wang X,Cheng Q,Liu J,Zhang L,Guo T,Liu C,Zheng H

Fri Jun 03 00:00:00 EDT 2016

A ubiquitin shuttle DC-UbP/UBTD2 reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes.

PloS one

Song AX,Yang H,Gao YG,Zhou CJ,Zhang YH,Hu HY

Thu Mar 31 00:00:00 EDT 2016

The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.

Blood

Zhang Z,Tong J,Tang X,Juan J,Cao B,Hurren R,Chen G,Taylor P,Xu X,Shi CX,Du J,Hou J,Wang G,Wu D,Stewart AK,Schimmer AD,Moran MF,Mao X

Thu Mar 31 00:00:00 EDT 2016

Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.

Molecular pain

Gadotti VM,Caballero AG,Berger ND,Gladding CM,Chen L,Pfeifer TA,Zamponi GW

Sat Mar 14 00:00:00 EDT 2015

Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.

Molecular pain

Gadotti VM,Caballero AG,Berger ND,Gladding CM,Chen L,Pfeifer TA,Zamponi GW

Sat Mar 14 00:00:00 EDT 2015

Ubiquitin-specific protease 5 is required for the efficient repair of DNA double-strand breaks.

PloS one

Nakajima S,Lan L,Wei L,Hsieh CL,Rapić-Otrin V,Yasui A,Levine AS

Wed Sep 17 00:00:00 EDT 2014

Selenoprotein S is involved in maintenance and transport of multiprotein complexes.

The Biochemical journal

Turanov AA,Shchedrina VA,Everley RA,Lobanov AV,Yim SH,Marino SM,Gygi SP,Hatfield DL,Gladyshev VN

Mon Sep 15 00:00:00 EDT 2014

Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins.

Biochemistry

Besche HC,Haas W,Gygi SP,Goldberg AL

Tue Mar 24 00:00:00 EDT 2009

Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.

The Journal of biological chemistry

Dayal S,Sparks A,Jacob J,Allende-Vega N,Lane DP,Saville MK

Fri Feb 20 00:00:00 EST 2009

Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.

The Journal of biological chemistry

Dayal S,Sparks A,Jacob J,Allende-Vega N,Lane DP,Saville MK

Fri Feb 20 00:00:00 EST 2009

Bioinformatics

Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; ubiquitin carboxy terminal hydrolase T; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5

Gene Aliases: AA407472; ISOT; ISOT-1; Ucht; USP5

UniProt ID: (Human) P45974, (Mouse) P56399

Entrez Gene ID: (Human) 8078, (Rat) 297593, (Mouse) 22225

Molecular Function: cysteine protease