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Product Details
PA5-28243
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 503 and 858 of Human USP5
Conjugate
Form
Liquid
Concentration
0.81 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545719
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain.^M
^M
Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (81%), Rhesus Monkey (92%), Bovine (99%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; ubiquitin carboxy terminal hydrolase T; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5
Gene Aliases: AA407472; ISOT; ISOT-1; Ucht; USP5
UniProt ID: (Human) P45974, (Mouse) P56399
Entrez Gene ID: (Human) 8078, (Mouse) 22225
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
zinc ion binding
ubiquitin binding
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
metal ion binding
cysteine protease
ubiquitin-dependent protein catabolic process
protein deubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
protein K48-linked deubiquitination
proteolysis
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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