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Bethyl Laboratories
USP5/IsoT Polyclonal Antibody
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FIGURE: 1 / 3
USP5/IsoT Antibody (IHC-00313) in IHC (P)
Detection of human USP5/IsoT by IHC-IF. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP5/IsoT (Product # IHC-00313) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus™ 555 used at a dilution of 1:100.
Product Details
IHC-00313
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 375 and 425 of Human ubiquitin specific peptidase 5 (isopeptidase T).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Amount
10 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Monkey, Gorilla, Chimpanzee, Northern white-cheeked gibbon, Crab-eating macaque and White-tufted-ear marmoset
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. It is involved in unanchored Lys-48-linked polyubiquitin disassembly, and also binds linear and Lys-63 linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme 5; Isopeptidase T; testicular tissue protein Li 218; Ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); Ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); Ubiquitin-specific-processing protease 5
Gene Aliases: ISOT; USP5
UniProt ID: (Human) P45974
Entrez Gene ID: (Human) 8078
negative regulation of T cell mediated immune response to tumor cell
proteolysis
regulation of mitotic cell cycle
protein ubiquitination
protein deubiquitination
regulation of protein stability
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of translation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of T cell activation
protein K48-linked deubiquitination
regulation protein catabolic process at presynapse
positive regulation of proteasomal protein catabolic process
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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