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Bethyl Laboratories
USP9X Polyclonal Antibody
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FIGURE: 1 / 1
USP9X Antibody (A301-351A) in WB
Detection of human and mouse USP9X by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USP9X antibody (Product # A301-351A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. USP9X was also immunoprecipitated by rabbit anti-USP9X antibody A301-350A, which recognizes an upstream epitope. Detection: Chemiluminescence with exp... View More
Product Details
A301-351A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 2497 and 2547 of Human ubiquitin specific peptidase 9, X-linked.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
USP9X is an ubiquitin-specific protease involved in the removal and recycling of ubiquitin from proteins degraded in proteasomes. As a deubiquitinating enzyme, USP9x has a critical function in tight junction biogenesis by controlling the levels of the exchange factor for Arf6 (EFA6), a protein shown to facilitate tight junction formation during a narrow temporal window preceding the establishment of cell polarity. USP9X is also located on the X-chromosome but escapes X-inactivation, and defects in USP9X may cause gonadal degeneration in Turner syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deubiquitinating enzyme FAF-X; Drosophila fat facets related, X-linked; Fat facets homolog; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; fat facets-like, X-linked; fats facets protein related, X; hFAM; probable ubiquitin carboxyl-terminal hydrolase FAF-X; similar to Homo sapiens ubiquitin hydrolase encoded by GenBank Accession Number X98296; Ubiquitin carboxyl-terminal hydrolase 9X; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific protease 9 SSSRF- isoform; ubiquitin specific protease 9, X chromosome; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); Ubiquitin thioesterase FAF-X; ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; unnamed protein product
Gene Aliases: 5730589N07Rik; AA407302; AA407699; AL022658; AL022749; DFFRX; FAF; FAF-X; Fafl; FAM; hFAM; MRX99; MRXS99F; USP9; USP9X; XLID99
UniProt ID: (Human) Q93008, (Mouse) P70398
Entrez Gene ID: (Human) 8239, (Mouse) 22284
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
hydrolase activity
Lys63-specific deubiquitinase activity
co-SMAD binding
ubiquitinyl hydrolase activity
molecular sequestering activity
K11-linked deubiquitinase activity
Lys48-specific deubiquitinase activity
thiol-dependent ubiquitinyl hydrolase activity
cysteine protease
negative regulation of transcription from RNA polymerase II promoter
neuron migration
DNA dealkylation involved in DNA repair
proteolysis
chromosome segregation
transforming growth factor beta receptor signaling pathway
female gamete generation
protein localization
cell migration
protein import into peroxisome matrix
protein import into peroxisome matrix, receptor recycling
protein ubiquitination
protein deubiquitination
BMP signaling pathway
regulation of protein stability
positive regulation of protein binding
negative regulation of proteasomal ubiquitin-dependent protein catabolic process
monoubiquitinated protein deubiquitination
regulation of circadian rhythm
rhythmic process
axon extension
protein stabilization
cell division
cilium morphogenesis
cytosolic ciliogenesis
protein K63-linked deubiquitination
protein deubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of cilium assembly
positive regulation of TORC2 signaling
amyloid fibril formation
in utero embryonic development
ubiquitin-dependent protein catabolic process
cell cycle
mitotic nuclear division
post-embryonic development
cerebellar cortex structural organization
hippocampus development
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of proteasomal protein catabolic process
cellular response to transforming growth factor beta stimulus
neuron projection extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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