Invitrogen

V-ATPase D Polyclonal Antibody

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V-ATPase D Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of V-ATPase D in human kidney, liver, lymph node and testis using V-ATPase D Polyclonal Antibody (Product # PA5-56767) (A) shows similar protein distribution across tissues to an independent V-ATPase D Polyclonal Antibody (B).

V-ATPase D Antibody in Western Blot (WB) — Western blot analysis of V-ATPase D in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a V-ATPase D Polyclonal Antibody (Product # PA5-56767).

Product Details

PA5-56767

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human V-ATPase D View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2649403

Product Specific Information

Immunogen sequence: VNAIEHVIIP RIERTLAYII TELDEREREE FYRLKKIQEK KKILKEKSEK DLEQRRAAGE VLEPANLLAE

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 96%.

Target Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, H+ transporting lysosomal (vacuolar proton pump); ATPase, H+ transporting lysosomal, member M; ATPase, H+ transporting, lysosomal (vacuolar proton pump); ATPase, H+ transporting, lysosomal 34kD, V1 subunit D; ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D; ATPase, H+ transporting, V1 subunit D; H(+)-transporting two-sector ATPase, subunit M; lysosomal 34kDa; V-ATPase 28 kDa accessory protein; V-ATPase D subunit; V-ATPase subunit D; V-type proton ATPase subunit D; vac; vacuolar ATP synthase subunit D; vacuolar H-ATPase subunit D; vacuolar proton pump D subunit; vacuolar proton pump delta polypeptide; Vacuolar proton pump subunit D; vacuolar proton-ATPase subunit D

Gene Aliases: 1110004P10Rik; ATP6M; ATP6V1D; VATD; VMA8

UniProt ID: (Human) Q9Y5K8, (Mouse) P57746

Entrez Gene ID: (Human) 51382, (Rat) 299159, (Mouse) 73834

Molecular Function: ATP synthase