Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.04-0.4 µg/mL | - |
Immunohistochemistry (IHC) |
1:500-1:1,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human V-ATPase D |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.3 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2649403 |
Immunogen sequence: VNAIEHVIIP RIERTLAYII TELDEREREE FYRLKKIQEK KKILKEKSEK DLEQRRAAGE VLEPANLLAE
Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 96%.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATPase, H+ transporting lysosomal (vacuolar proton pump); ATPase, H+ transporting lysosomal, member M; ATPase, H+ transporting, lysosomal (vacuolar proton pump); ATPase, H+ transporting, lysosomal 34kD, V1 subunit D; ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D; ATPase, H+ transporting, V1 subunit D; H(+)-transporting two-sector ATPase, subunit M; lysosomal 34kDa; V-ATPase 28 kDa accessory protein; V-ATPase D subunit; V-ATPase subunit D; V-type proton ATPase subunit D; vac; vacuolar ATP synthase subunit D; vacuolar H-ATPase subunit D; vacuolar proton pump D subunit; vacuolar proton pump delta polypeptide; Vacuolar proton pump subunit D; vacuolar proton-ATPase subunit D
Gene Aliases: 1110004P10Rik; ATP6M; ATP6V1D; VATD; VMA8
UniProt ID: (Human) Q9Y5K8, (Mouse) P57746
Entrez Gene ID: (Human) 51382, (Rat) 299159, (Mouse) 73834
Molecular Function:
ATP synthase
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