|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from the C-terminal of rat VAChT conjugated to BSA.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:800 - 1:1200|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For immunohistochemistry (paraffin) applications, it is recommended to microwave samples prior to staining. For flow cytometry, incubate overnight at 4°C.
Reconstitute with 50-100 ul of distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.1% sodium azide. For long-term storage, aliquot and store at -20°C.
VACHt is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.