|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.1-1 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S A P A Q P P A E G T E G A A P G(18) C of rat VAMP-1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-765 detects vesicle associated membrane protein 1 (VAMP-1) from human, canine, mouse and rat tissues.
PA1-765 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 15 kDa protein representing VAMP-1 from rat brain whole protein extract.
The PA1-765 immunogen is a synthetic peptide corresponding to residues M(1) S A P A Q P P A E G T E G A A P G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-099) is available for use in neutralization and control experiments.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer and quote;s disease, VAMP levels of all isoforms appear to be significantly lowered. It has been shown that the extreme carboxy-terminus of VAMP-1 is involved in subcellular vesicle targeting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: synaptobrevin 1; Synaptobrevin-1; VAMP-1; Vesicle Associated Membrane Protein 1; Vesicle-associated membrane protein 1; vesicle-associated membrane protein 1 (synaptobrevin 1)
Gene Aliases: lew; SPAX1; Syb-1; SYB1; VAMP-1; VAMP1
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