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Western blot analysis was performed on tissues (40 ug lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2) and Rat Brain (Lane 3). The blots were probed with Anti-VAMP2 Rabbit Polyclonal Antibody (Product# PA1766, 0.1-1 ug/ml) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution). A 13 kDa band corresponding to VAMP2 was observed across the tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-766 detects vesicle associated membrane protein 2 (VAMP-2) from rat and mouse tissues.
PA1-766 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19 kDa protein representing VAMP-2 from rat brain whole protein extract.
The PA1-766 immunogen is a synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-101) is available for use in neutralization and control experiments.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer"e;s disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.
PA1-766 was used in western blot to study the role of the Rab-GTPase-activating protein TBC1D1 in regulating skeletal muscle glucose metabolism
|Szekeres F,Chadt A,Tom RZ,Deshmukh AS,Chibalin AV,Björnholm M,Al-Hasani H,Zierath JR||American journal of physiology. Endocrinology and metabolism (303:E524)||2012|
Contraction-related stimuli regulate GLUT4 traffic in C2C12-GLUT4myc skeletal muscle cells.
PA1-766 was used in western blot to develop a cellular model for glucose uptake following muscle contraction
|Niu W,Bilan PJ,Ishikura S,Schertzer JD,Contreras-Ferrat A,Fu Z,Liu J,Boguslavsky S,Foley KP,Liu Z,Li J,Chu G,Panakkezhum T,Lopaschuk GD,Lavandero S,Yao Z,Klip A||American journal of physiology. Endocrinology and metabolism (298:E1058)||2010|
Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells.
PA1-766 was used in western blot to study the function ofa calpain-10 isoform as an exocytosis trigger in pancreatic beta-cells.
|Marshall C,Hitman GA,Partridge CJ,Clark A,Ma H,Shearer TR,Turner MD||Molecular endocrinology (Baltimore, Md.) (19:213)||2005|
|Not Applicable||Not Cited||
The septin CDCrel-1 is dispensable for normal development and neurotransmitter release.
PA1-766 was used in immunofluorescence to probe VAMP-2 in primary cultures of mouse hippocampal neurons.
|Peng XR,Jia Z,Zhang Y,Ware J,Trimble WS||Molecular and cellular biology (22:378)||2002|
synaptobrevin 2; Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; synaptobrevin-2; VAMP-2; Vesicle Associated Membrane Protein 2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); vesicle-associated membrane protein 2; vesicle-associated membrane protein 2 (synaptobrevin 2)
RATVAMPB; RATVAMPIR; SYB; Syb-2; SYB2; sybII; VAMP-2; VAMP2