Invitrogen

VAMP3 Polyclonal Antibody

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VAMP3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a VAMP3 polyclonal antibody (Product # PA1-767A) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.

VAMP3 Antibody in Western Blot (WB) — Western blot analysis of VAMP3 was performed by loading 25 µg of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a VAMP3 polyclonal antibody (Product # PA1-767A) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~13 kDa.

VAMP3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP3 was observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using VAMP3 Polyclonal Antibody (Product # PA1-767A) in western blot. {RE}

Product Details

PA1-767A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:5,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2288259

Product Specific Information

PA1-767A detects vesicle-associated membrane protein 3 (VAMP-3)/cellubrevin in human, rat and mouse samples.

PA1-767A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~13 kDa protein representing VAMP-3 in HeLa cell lysates.

PA1-767A immunogen is a sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3. This immunizing peptide (Cat. # PEP-145) is available for use in neutralization and control experiments.

Target Information

Synaptobrevins/VAMPs, syntaxins, and the 25-kDa synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells.

Journal of cell science

Fujii S,Kurokawa K,Inaba R,Hiramatsu N,Tago T,Nakamura Y,Nakano A,Satoh T,Satoh AK

PA1-767A was used in Immunocytochemistry to indicate that fly and mammalian recycling endosomes are organelles that are equivalent to trans-Golgi networks in plants.

Wed Feb 26 00:00:00 EST 2020

SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.

Molecular biology of the cell

Williams KC,McNeilly RE,Coppolino MG

PA1-767A was used in western blot to study the trafficking of MT1-MMP to invadopodia during tumor invasion and the roles played by SNAP23, syntaxin-4 and VAMP7

Tue Jul 01 00:00:00 EDT 2014

Characterization of VAMP-2 in the lung: implication in lung surfactant secretion.

Cell biology international

Wang P,Howard MD,Zhang H,Chintagari NR,Bell A,Jin N,Mishra A,Liu L

PA1-767A was used in Western Blotting to characterise the distribution of vesicle-associated membrane proteins in rat lung and alveolar type II cells.

Sat Sep 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CEB; Cellubrevin; Synaptobrevin-3; vamp; VAMP-3; Vesicle-associated membrane protein 3

Gene Aliases: CEB; D130027G05Rik; SYB3; VAMP-3; VAMP3

UniProt ID: (Human) Q15836, (Mouse) P63024, (Rat) P63025

Entrez Gene ID: (Human) 9341, (Mouse) 22319, (Rat) 29528

Function(s)

positive regulation of receptor recycling protein complex assembly transport vesicle fusion protein transport vesicle-mediated transport calcium ion regulated exocytosis substrate adhesion-dependent cell spreading SNARE complex assembly retrograde transport, endosome to Golgi Golgi to plasma membrane protein transport negative regulation of secretion by cell exocytosis vesicle docking involved in exocytosis positive regulation of immunoglobulin secretion membrane fusion mucus secretion regulation of histamine secretion by mast cell neurotransmitter secretion

Process(es)

SNARE binding SNAP receptor activity protein binding syntaxin-1 binding

VAMP3 Polyclonal Antibody

Product Details

PA1-767A

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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