|Western Blot (WB)||1:1000-1:5000|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-767A detects vesicle-associated membrane protein 3 (VAMP-3)/cellubrevin in human, rat and mouse samples.
PA1-767A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~13 kDa protein representing VAMP-3 in HeLa cell lysates.
PA1-767A immunogen is a sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3. This immunizing peptide (Cat. # PEP-145) is available for use in neutralization and control experiments.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer and quote;s disease, all VAMP isoform levels appear to be significantly reduced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CEB; Cellubrevin; Synaptobrevin-3; VAMP-3; Vesicle Associated Membrane Protein 3; Vesicle-associated membrane protein 3
Gene Aliases: CEB; D130027G05Rik; SYB3; VAMP-3; VAMP3
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