This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details
PA1-768 detects VAMP-4 from human and mouse samples.
PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining.
Immunofluorescence staining with this antibody requires saponin permeabilization.
The PA1-768 antigen is recombinant rat VAMP-4.
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: vamp; VAMP-4; vesicle associated membrane protein 4; Vesicle-associated membrane protein 4
Gene Aliases: D1Ertd147e; VAMP-4; VAMP24; VAMP4