|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit|
|Immunogen||A synthetic peptide from aa region 150-100 of human Vesicle-associated membrane protein 7|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1:300 - 1:2000|
|Western Blot (WB)||1:300 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glycerol (1:1) may be added for additional stability.
Reconstitute in 100ul of sterile water.
Exocytosis of vesicle contents requires fusion of opposing membrane layers, and the highly conserved proteins of the SNARE family are crucially involved in this process. SNAREs localized to the vesicle membrane, or v-SNAREs, include VAMP7, and SNAREs localized to the target membrane, or t-SNARES, include the SNAPs (see SNAP25; MIM 600322) and the syntaxins (see STX1A; MIM 186590). The SNARE proteins assemble into a core complex of bundled helices and thereby contribute to membrane fusion (Sander et al., 2008 [PubMed 18253931]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.