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Rockland
VARP Polyclonal Antibody
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FIGURE: 1 / 1
VARP Antibody (600-401-FV9) in IHC (P)
Immunohistochemistry of VARP antibody. Tissue: Human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: VARP antibody at 5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: VARP is a multi-pass membrane protein. Staining: VARP as precipitated pink signal with blue nuclear cou... View More
Product Details
600-401-FV9
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-VARP antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide near the internal region of human VARP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ankyrin repeat domain 27 (VPS9 domain); Ankyrin repeat domain-containing protein 27; Vps9 domain and ankyrin-repeat-containing protein; VPS9 domain-containing protein; VPS9-ankyrin-repeat protein
Gene Aliases: ANKRD27; PP12899; VARP
UniProt ID: (Human) Q96NW4
Entrez Gene ID: (Human) 84079
protein transport
negative regulation of SNARE complex assembly
endosome to melanosome transport
positive regulation of GTPase activity
early endosome to late endosome transport
neuron projection morphogenesis
positive regulation of dendrite morphogenesis
retrograde transport, endosome to plasma membrane
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